Automatically showing microbial growth kinetics with a high-performance microbial growth analyzer

It is difficult to show microbial growth kinetics online when they grow in complex matrices. We presented a novel strategy to address this challenge by developing a high-performance microbial growth analyzer (HPMGA), which employed a unique 32-channel capacitively coupled contactless conductivity detector as a sensing element and fixed with a CellStatz software. It was capable of online showing accurate and repeatable growth curves of well-dispersed and bad-dispersed microbes, whether they grew in homogeneous simple culture broth or heterogeneous complex matrices. Moreover, it could automatically report key growth kinetics parameters. In comparison to optical density (OD), plate counting and broth microdilution (BMD) methods, we demonstrated its practicability in five scenarios: 1) the illustration of the growth, growth rate, and acceleration curves of Escherichia coli (E. coli); 2) the antimicrobial susceptibility testing (AST) of Oxacillin against Staphylococcus aureus (S. aureus); 3) the determination of Ag nanoparticle toxicity on Providencia rettgeri (P. rettgeri); 4) the characterization of milk fermentation; and 5) the enumeration of viable pathogenic Vibrio in shrimp body. Results highlighted that the HPMGA method had the advantages of universality and effectivity. This technology would significantly facilitate the routine analysis of microbial growth in many fields (biology, medicine, clinic, life, food, environment, and ecology), paving an avenue for microbiologists to achieve research goals that have been inhibited for years due to a lack of practical analytical methods. Based on a unique 32-channel capacitively coupled contactless conductivity detector and novel algorithms, the high-performance microbial growth analyzer (HPMGA) was capable of online showing the growth curves of well-dispersed and bad-dispersed microorganisms (e.g. E. coli, S. aureus, L. bulgaricus, P. rettgeri, and pathogenic Vibrio (flora)), whether they grew in homogeneous simple culture broth or heterogeneous complex matrices (e.g. blood, sewage sludge, milk, and shrimp bodies). Moreover, it could decompose the growth curves into growth rate and growth acceleration curves, and report key growth kinetics parameters. [Display omitted]