Effect of electron beam irradiation on raw goat milk: microbiological, physicochemical and protein structural analysis
BACKGROUND Goat milk is considered a nutritionally superior resource, owing to its advantageous nutritional attributes. Nevertheless, it is susceptible to spoilage and the persistence of pathogens. Electron beam irradiation stands as a promising non‐thermal processing technique capable of prolonging...
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| Veröffentlicht in: | Journal of the science of food and agriculture 2024-09, Vol.104 (12), p.7713-7721 |
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| creator | Wen, Chunlu Peng, Yue Zhang, Linlu Chen, Ya Yu, Jiangtao Bai, Junqing Yang, Kui Ding, Wu |
| description | BACKGROUND
Goat milk is considered a nutritionally superior resource, owing to its advantageous nutritional attributes. Nevertheless, it is susceptible to spoilage and the persistence of pathogens. Electron beam irradiation stands as a promising non‐thermal processing technique capable of prolonging shelf life with minimal residue and a high degree of automation.
RESULTS
The effects of electron beam irradiation (2, 3, 5, and 7 kGy) on microorganisms, physicochemical properties, and protein structure of goat milk compared with conventional pasteurized goat milk (PGM) was evaluated. It was found that a 2 kGy electron beam irradiation reduces the total microbial count of goat milk by 6‐logs, and the irradiated goat milk protein secondary structure showed a significant decrease in ɑ‐helix content. Low irradiation doses led to microaggregation and crosslinking. In contrast, high doses (≥ 5 kGy) slightly disrupted the aggregates and decreased the particle size, disrupting the microscopic surface structure of goat milk, verified by scanning electron microscopy and confocal laser scanning microscopy.
CONCLUSION
The irradiation of goat milk with a 2 kGy electron beam may effectively inactivate harmful microorganisms in the milk and maintain/or improve the physicochemical quality and protein structure of goat milk compared to thermal pasteurization. © 2024 Society of Chemical Industry. |
| doi_str_mv | 10.1002/jsfa.13518 |
| format | Article |
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Goat milk is considered a nutritionally superior resource, owing to its advantageous nutritional attributes. Nevertheless, it is susceptible to spoilage and the persistence of pathogens. Electron beam irradiation stands as a promising non‐thermal processing technique capable of prolonging shelf life with minimal residue and a high degree of automation.
RESULTS
The effects of electron beam irradiation (2, 3, 5, and 7 kGy) on microorganisms, physicochemical properties, and protein structure of goat milk compared with conventional pasteurized goat milk (PGM) was evaluated. It was found that a 2 kGy electron beam irradiation reduces the total microbial count of goat milk by 6‐logs, and the irradiated goat milk protein secondary structure showed a significant decrease in ɑ‐helix content. Low irradiation doses led to microaggregation and crosslinking. In contrast, high doses (≥ 5 kGy) slightly disrupted the aggregates and decreased the particle size, disrupting the microscopic surface structure of goat milk, verified by scanning electron microscopy and confocal laser scanning microscopy.
CONCLUSION
The irradiation of goat milk with a 2 kGy electron beam may effectively inactivate harmful microorganisms in the milk and maintain/or improve the physicochemical quality and protein structure of goat milk compared to thermal pasteurization. © 2024 Society of Chemical Industry.</description><identifier>ISSN: 0022-5142</identifier><identifier>ISSN: 1097-0010</identifier><identifier>EISSN: 1097-0010</identifier><identifier>DOI: 10.1002/jsfa.13518</identifier><identifier>PMID: 38591367</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>agriculture ; Automation ; Confocal microscopy ; Crosslinking ; electron beam irradiation ; Electron beams ; Electron irradiation ; electron microscopy ; goat milk ; Goat's milk ; Goats ; Irradiation ; Laser radiation ; microbial inactivation ; Microorganisms ; Microscopy ; Milk ; milk proteins ; particle size ; Pasteurization ; Physicochemical properties ; protein secondary structure ; Protein structure ; Proteins ; Scanning electron microscopy ; Scanning microscopy ; Secondary structure ; Shelf life ; Spoilage ; Structural analysis ; Surface structure</subject><ispartof>Journal of the science of food and agriculture, 2024-09, Vol.104 (12), p.7713-7721</ispartof><rights>2024 Society of Chemical Industry.</rights><rights>This article is protected by copyright. All rights reserved.</rights><rights>2024 Society of Chemical Industry</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3498-73f841412299d70457baf061e668146ba8884992965610c5d02c39121f848b1d3</cites><orcidid>0009-0003-4619-6328 ; 0000-0002-7360-2147</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjsfa.13518$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjsfa.13518$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38591367$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wen, Chunlu</creatorcontrib><creatorcontrib>Peng, Yue</creatorcontrib><creatorcontrib>Zhang, Linlu</creatorcontrib><creatorcontrib>Chen, Ya</creatorcontrib><creatorcontrib>Yu, Jiangtao</creatorcontrib><creatorcontrib>Bai, Junqing</creatorcontrib><creatorcontrib>Yang, Kui</creatorcontrib><creatorcontrib>Ding, Wu</creatorcontrib><title>Effect of electron beam irradiation on raw goat milk: microbiological, physicochemical and protein structural analysis</title><title>Journal of the science of food and agriculture</title><addtitle>J Sci Food Agric</addtitle><description>BACKGROUND
Goat milk is considered a nutritionally superior resource, owing to its advantageous nutritional attributes. Nevertheless, it is susceptible to spoilage and the persistence of pathogens. Electron beam irradiation stands as a promising non‐thermal processing technique capable of prolonging shelf life with minimal residue and a high degree of automation.
RESULTS
The effects of electron beam irradiation (2, 3, 5, and 7 kGy) on microorganisms, physicochemical properties, and protein structure of goat milk compared with conventional pasteurized goat milk (PGM) was evaluated. It was found that a 2 kGy electron beam irradiation reduces the total microbial count of goat milk by 6‐logs, and the irradiated goat milk protein secondary structure showed a significant decrease in ɑ‐helix content. Low irradiation doses led to microaggregation and crosslinking. In contrast, high doses (≥ 5 kGy) slightly disrupted the aggregates and decreased the particle size, disrupting the microscopic surface structure of goat milk, verified by scanning electron microscopy and confocal laser scanning microscopy.
CONCLUSION
The irradiation of goat milk with a 2 kGy electron beam may effectively inactivate harmful microorganisms in the milk and maintain/or improve the physicochemical quality and protein structure of goat milk compared to thermal pasteurization. © 2024 Society of Chemical Industry.</description><subject>agriculture</subject><subject>Automation</subject><subject>Confocal microscopy</subject><subject>Crosslinking</subject><subject>electron beam irradiation</subject><subject>Electron beams</subject><subject>Electron irradiation</subject><subject>electron microscopy</subject><subject>goat milk</subject><subject>Goat's milk</subject><subject>Goats</subject><subject>Irradiation</subject><subject>Laser radiation</subject><subject>microbial inactivation</subject><subject>Microorganisms</subject><subject>Microscopy</subject><subject>Milk</subject><subject>milk proteins</subject><subject>particle size</subject><subject>Pasteurization</subject><subject>Physicochemical properties</subject><subject>protein secondary structure</subject><subject>Protein structure</subject><subject>Proteins</subject><subject>Scanning electron microscopy</subject><subject>Scanning microscopy</subject><subject>Secondary structure</subject><subject>Shelf life</subject><subject>Spoilage</subject><subject>Structural analysis</subject><subject>Surface structure</subject><issn>0022-5142</issn><issn>1097-0010</issn><issn>1097-0010</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkU9v1DAQxS1ERZfChQ-ALHFBqCkz8Z_Y3KqqLVSVegDOkeM4rRdnvdgJ1X57nG7hwIFK1th6_s2Tx4-QNwgnCFB_XOfBnCATqJ6RFYJuKgCE52RVLutKIK8Pycuc1wCgtZQvyCFTQiOTzYr8Oh8GZycaB-pCOaS4oZ0zI_Upmd6byRehrGTu6W00Ex19-PGpVJti52OIt96acEy3d7vsbbR3blwEajY93aY4Ob-heUqzneb0IJtQwPyKHAwmZPf6cT8i3y_Ov519rq5vLr-cnV5XlnGtqoYNiiPHuta6b4CLpjMDSHRSKuSyM0oprnWtpZAIVvRQW6axxtKmOuzZEXm_9y1v-Tm7PLWjz9aFYDYuzrllKJjiUgt4GgUmoOG6XtB3_6DrOKcy2kIpDZJLZIX6sKfKV-Wc3NBukx9N2rUI7RJcuwTXPgRX4LePlnM3uv4v-iepAuAeuPfB7f5j1V59vTjdm_4GLBahug</recordid><startdate>202409</startdate><enddate>202409</enddate><creator>Wen, Chunlu</creator><creator>Peng, Yue</creator><creator>Zhang, Linlu</creator><creator>Chen, Ya</creator><creator>Yu, Jiangtao</creator><creator>Bai, Junqing</creator><creator>Yang, Kui</creator><creator>Ding, Wu</creator><general>John Wiley & Sons, Ltd</general><general>John Wiley and Sons, Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QL</scope><scope>7QQ</scope><scope>7QR</scope><scope>7SC</scope><scope>7SE</scope><scope>7SN</scope><scope>7SP</scope><scope>7SR</scope><scope>7ST</scope><scope>7T5</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>M7N</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0009-0003-4619-6328</orcidid><orcidid>https://orcid.org/0000-0002-7360-2147</orcidid></search><sort><creationdate>202409</creationdate><title>Effect of electron beam irradiation on raw goat milk: microbiological, physicochemical and protein structural analysis</title><author>Wen, Chunlu ; Peng, Yue ; Zhang, Linlu ; Chen, Ya ; Yu, Jiangtao ; Bai, Junqing ; Yang, Kui ; Ding, Wu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3498-73f841412299d70457baf061e668146ba8884992965610c5d02c39121f848b1d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>agriculture</topic><topic>Automation</topic><topic>Confocal microscopy</topic><topic>Crosslinking</topic><topic>electron beam irradiation</topic><topic>Electron beams</topic><topic>Electron irradiation</topic><topic>electron microscopy</topic><topic>goat milk</topic><topic>Goat's milk</topic><topic>Goats</topic><topic>Irradiation</topic><topic>Laser radiation</topic><topic>microbial inactivation</topic><topic>Microorganisms</topic><topic>Microscopy</topic><topic>Milk</topic><topic>milk proteins</topic><topic>particle size</topic><topic>Pasteurization</topic><topic>Physicochemical properties</topic><topic>protein secondary structure</topic><topic>Protein structure</topic><topic>Proteins</topic><topic>Scanning electron microscopy</topic><topic>Scanning microscopy</topic><topic>Secondary structure</topic><topic>Shelf life</topic><topic>Spoilage</topic><topic>Structural analysis</topic><topic>Surface structure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wen, Chunlu</creatorcontrib><creatorcontrib>Peng, Yue</creatorcontrib><creatorcontrib>Zhang, Linlu</creatorcontrib><creatorcontrib>Chen, Ya</creatorcontrib><creatorcontrib>Yu, Jiangtao</creatorcontrib><creatorcontrib>Bai, Junqing</creatorcontrib><creatorcontrib>Yang, Kui</creatorcontrib><creatorcontrib>Ding, Wu</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Ceramic Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Ecology Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Journal of the science of food and agriculture</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wen, Chunlu</au><au>Peng, Yue</au><au>Zhang, Linlu</au><au>Chen, Ya</au><au>Yu, Jiangtao</au><au>Bai, Junqing</au><au>Yang, Kui</au><au>Ding, Wu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of electron beam irradiation on raw goat milk: microbiological, physicochemical and protein structural analysis</atitle><jtitle>Journal of the science of food and agriculture</jtitle><addtitle>J Sci Food Agric</addtitle><date>2024-09</date><risdate>2024</risdate><volume>104</volume><issue>12</issue><spage>7713</spage><epage>7721</epage><pages>7713-7721</pages><issn>0022-5142</issn><issn>1097-0010</issn><eissn>1097-0010</eissn><abstract>BACKGROUND
Goat milk is considered a nutritionally superior resource, owing to its advantageous nutritional attributes. Nevertheless, it is susceptible to spoilage and the persistence of pathogens. Electron beam irradiation stands as a promising non‐thermal processing technique capable of prolonging shelf life with minimal residue and a high degree of automation.
RESULTS
The effects of electron beam irradiation (2, 3, 5, and 7 kGy) on microorganisms, physicochemical properties, and protein structure of goat milk compared with conventional pasteurized goat milk (PGM) was evaluated. It was found that a 2 kGy electron beam irradiation reduces the total microbial count of goat milk by 6‐logs, and the irradiated goat milk protein secondary structure showed a significant decrease in ɑ‐helix content. Low irradiation doses led to microaggregation and crosslinking. In contrast, high doses (≥ 5 kGy) slightly disrupted the aggregates and decreased the particle size, disrupting the microscopic surface structure of goat milk, verified by scanning electron microscopy and confocal laser scanning microscopy.
CONCLUSION
The irradiation of goat milk with a 2 kGy electron beam may effectively inactivate harmful microorganisms in the milk and maintain/or improve the physicochemical quality and protein structure of goat milk compared to thermal pasteurization. © 2024 Society of Chemical Industry.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>38591367</pmid><doi>10.1002/jsfa.13518</doi><tpages>9</tpages><orcidid>https://orcid.org/0009-0003-4619-6328</orcidid><orcidid>https://orcid.org/0000-0002-7360-2147</orcidid></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | agriculture Automation Confocal microscopy Crosslinking electron beam irradiation Electron beams Electron irradiation electron microscopy goat milk Goat's milk Goats Irradiation Laser radiation microbial inactivation Microorganisms Microscopy Milk milk proteins particle size Pasteurization Physicochemical properties protein secondary structure Protein structure Proteins Scanning electron microscopy Scanning microscopy Secondary structure Shelf life Spoilage Structural analysis Surface structure |
| title | Effect of electron beam irradiation on raw goat milk: microbiological, physicochemical and protein structural analysis |
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