GW3, encoding a member of the P450 subfamily, controls grain width by regulating the GA4 content in spikelets of rice (Oryza sativa L.)
Key message A stable QTL, GW3 , controlling grain width was identified in two populations. Its causal gene LOC_Os03g04680 was verified by gene-based haplotype analysis, expression analysis, gene knockout and complementation transgenic tests. Grain width (GW) is one of the key traits affecting grain...
Gespeichert in:
Veröffentlicht in: | Theoretical and applied genetics 2024-11, Vol.137 (11), p.251-251, Article 251 |
---|---|
Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Key message
A stable QTL,
GW3
, controlling grain width was identified in two populations. Its causal gene
LOC_Os03g04680
was verified by gene-based haplotype analysis, expression analysis, gene knockout and complementation transgenic tests.
Grain width (GW) is one of the key traits affecting grain size and determines grain yield and appearance quality in rice. Mining gene loci and elite alleles controlling GW is necessary. The GW phenotypes of the two populations were investigated in three environments, which showed abundant phenotypic variation.
GW3,
encoding a P450 subfamily protein, was identified and validated as a causal gene by gene-based haplotype analysis, expression analysis, gene knockout and complementation transgenic tests. The accessions with large GW values had high gene expression levels. In addition, the GW of the accessions with the GG allele was significantly greater than that of the accessions with the AA allele. The Hap 1 and Hap 3 were identified as elite haplotypes, which can increase GW. The expression levels of
OsKO1
,
OsGA3ox1
,
OsGA20ox1
and
OsGA20ox2
in the young panicle of A7444 were significantly greater than those in the young panicle of the mutants, indicating that
GW3
may be involved in the gibberellins (GA) biosynthesis pathway to regulate GW. GA
4
content detection and electron scanning analysis revealed that GA
4
regulates GW by affecting glume cell size. These results provide new insights for studying the genetic mechanism of rice GW and provide a material basis for breeding high-yield rice varieties. |
---|---|
ISSN: | 0040-5752 1432-2242 1432-2242 |
DOI: | 10.1007/s00122-024-04751-5 |