Behavioral, electrocardiographic, and opercular beat recording characterization of tilapia (Oreochromis niloticus) in immersion bath with different concentrations of tricaine (MS-222)

In the world scenario of farmed fish production, tilapia stands out as one of the most cultivated species. In view of animal welfare in fish farming, stressful practices such as biometrics and transportation are minimized by the use of anesthetic agents, with tricaine (MS-222) being among the most c...

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Veröffentlicht in:Aquaculture 2025-02, Vol.596, p.741700, Article 741700
Hauptverfasser: Reis, Thaysa de Sousa, da Paz, Clarissa Araújo, Quirino, Luciana Eiró, de Araújo, Daniella Bastos, Barbosa, Gabriela Brito, Santos, Rafaela Negrão Olivia, de Campos, Ana Luiza Cordeiro, Amoras, Laís Helena Baptista, Brito, Marcelo Victor dos Santos, Hamoy, Moisés
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Sprache:eng
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Zusammenfassung:In the world scenario of farmed fish production, tilapia stands out as one of the most cultivated species. In view of animal welfare in fish farming, stressful practices such as biometrics and transportation are minimized by the use of anesthetic agents, with tricaine (MS-222) being among the most commonly used. Despite its extensive use, its anesthetic responses remain a focus of investigation for animal safety. Therefore, the present study aims to evaluate the electrocardiographic and respiratory activity of juvenile Nile tilapia exposed to different concentrations of tricaine. In this study, juvenile Nile tilapia (30.9 ± 5.5 g, n = 99) were subjected to tricaine anesthetic baths at concentrations of 150–250 ppm for behavioral analysis and electrophysiological recordings (electrocardiogram and opercular beating). Our results show that induction and recovery of anesthesia with tricaine in juvenile Nile tilapia was effective for a short-term anesthesia with dose-dependence characteristics. Furthermore, the electrophysiological data reveal a marked decrease in heart rate (28.10–44.08 %) and opercular beating (35.72–59.19 %), depending on the concentration of tricaine used, with reversibility during the recovery period. Therefore, we can infer that the safe utilization window for tricaine varies between 150–250 ppm in juvenile tilapia, emphasizing that its administration should consider the anesthetic plan with monitoring of physiological parameters, especially respiratory ones, which are more easily evidenced. •Tricaine induces rapid anesthesia and recovery in O. niloticus.•Electrophysiological data reveal a decrease in heart rate and opercular beat frequency.•The safe utilization window of tricaine ranges between 150–250 ppm in juvenile tilapia.
ISSN:0044-8486
DOI:10.1016/j.aquaculture.2024.741700