Regulatory effects of non-growing season precipitation on the community structure, biomass allocation, and water-carbon utilization in a temperate desert steppe

•Changes in non-growing season precipitation promoted the dominant species dominance.•Non-growing season precipitation changed plant biomass allocation.•Non-growing season precipitation regulated plant water and carbon activity intensity.•Non-growing season precipitation affected plant water and carbon utilization strategy. Under the influence of global change, precipitation amounts and extreme precipitation frequency during non-growing seasons in mid-high latitude grasslands have been increasing. However, the ecological effects of non-growing season precipitation in the desert steppe have long been overlooked due to an insufficient understanding of the correlative mechanisms linking non-growing season precipitation to plant growth. Therefore, a 3-year non-growing season precipitation manipulation experiment was conducted to reveal the response of desert steppe plants to non-growing season precipitation changes. Our study indicates that, by influencing water budget and availability, non-growing season precipitation directly or indirectly impacted community structure, plant biomass allocation, and water-carbon utilization intensity. Adaptive strategies of communities and plants included: ① Dominant species enhanced their dominance in the community to adapt to non-growing season precipitation changes. ② Stipa krylovii exhibited different biomass allocation strategies in response to non-growing season precipitation variations. Plants in the precipitation shading plots tended to allocate biomass to the roots, while those in the precipitation increase plots favored aboveground development. ③ Persistent drought during the growing season intensified early insufficient development of plants in the precipitation shading plots. Upon entering the wet period, plants in the precipitation shading plots shifted into a compensatory growth mode with high water-carbon activity intensity, while those in the precipitation increase plots entered a moderate growth mode with relatively low water-carbon activity intensity. Additionally, our study found that the regulatory effects of non-growing season precipitation were more pronounced in the growing seasons with less precipitation in the early to middle stage. Moreover, increased non-growing season precipitation enhanced plant water use efficiency (WUE) and strengthened their resilience to drought conditions. Our study suggests that the ecological role of non-growing season precipitation may be further highlighted in the future