Comprehensive analytics for virus-cell and cell-cell multinucleation system

Cell-fusion mediated generation of multinucleated syncytia represent critical feature during viral infection and in development. Efficiency of syncytia formation is usually illustrated as fusion efficiency under given condition by quantifying total number of nuclei in syncytia normalized to total nu...

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Veröffentlicht in:Biochemical and biophysical research communications 2024-09, Vol.726, p.150281, Article 150281
Hauptverfasser: Kushwaha, Nisha, Dwivedi, Aditi, Tiwari, Swasti, Mishra, Prabhaker, Verma, Santosh Kumar
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Sprache:eng
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Zusammenfassung:Cell-fusion mediated generation of multinucleated syncytia represent critical feature during viral infection and in development. Efficiency of syncytia formation is usually illustrated as fusion efficiency under given condition by quantifying total number of nuclei in syncytia normalized to total number of nuclei (both within syncytia and unfused cell nuclei) in unit field of view. However heterogeneity in multinucleated syncytia sizes poses challenge in quantification of cell-fusion multinucleation under diverse conditions. Taking in-vitro SARS-CoV-2 spike-protein variants mediated virus-cell fusion model and placenta trophoblast syncytialization as cell-cell fusion model; herein we emphasize wide application of simple unbiased detailed measure of virus-cell and cell-cell multinucleation using experiential cumulative distribution function (CDF) and fusion number events (FNE) approaches illustrating comprehensive metrics for syncytia interpretation. •Multinucleated syncytia formation is an important event during virus infection and in placenta development.•Combination of fusion number events (FNE) and cumulative distribution function (CDF) define syncytia sizes.•FNE and CDF represent unbiased approach for syncytia interpretation both in virus-cell and cell-fusion models.
ISSN:0006-291X
1090-2104
1090-2104
DOI:10.1016/j.bbrc.2024.150281