Short-term colonization of fouling communities within the port of Livorno (Northern Tyrrhenian Sea, Western Mediterranean): Influence of substrate three-dimensional complexity on non-indigenous species establishment

The influence of substrate morphology on early stages of fouling development was assessed through submerged experimental substrates with different morphological complexity. The experiment was carried out within commercial and touristic harbours of the port of Livorno (Italy), analysing the communities at three steps of colonization (14, 28, 42 days). We assessed the effect of substrate complexity on recruitment of non-indigenous species (NIS), combined with the influence of port use destinations. NIS were recorded in both use destination areas since the first step of colonization. Substrate morphological complexity significantly affected fouling colonization and particularly NIS assemblages. We found that high-complexity substrates are particularly suitable for NIS establishment in comparison with less complex ones. The touristic harbour exhibited a potential for fouling colonization higher than the commercial harbour. These results contributed to the understanding of factors involved in NIS establishment and spread, as well as in their spatial-temporal dynamics within port environments. [Display omitted] •The early development of fouling was studied in a Mediterranean port.•Spatial heterogeneity related to use destinations was found in fouling development.•Substrates 3D morphology affected the fouling development.•High-complexity substrate supported higher occurrence of NIS.