Genomic analysis and metabolic characteristics provide insights into inorganic nitrogen metabolism of novel bacterium Acinetobacter pittii J08
[Display omitted] •Novel HN-AD bacterium, A. pittii J08, was isolated from pond sediments.•High removals of inorganic N and TN-N were analyzed in different N conditions.•Whole genome sequence and N metabolic pathways were analyzed.•Strain J08 showed a high adaptability to various culture conditions....
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| Veröffentlicht in: | Bioresource technology 2024-09, Vol.408, p.131228, Article 131228 |
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| creator | Ou, Jie Xie, Qing Zhong, Zi-Rou Wang, Fei Huang, Ming-Zhu Fang, Zi-Xuan Kuang, Xu-Ying Qin, Zi-Le Luo, Sheng-Wei |
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•Novel HN-AD bacterium, A. pittii J08, was isolated from pond sediments.•High removals of inorganic N and TN-N were analyzed in different N conditions.•Whole genome sequence and N metabolic pathways were analyzed.•Strain J08 showed a high adaptability to various culture conditions.
A novel A. pittii J08 with heterotrophic nitrification and aerobic denitrification (HN-AD) isolated from pond sediments could rapidly degrade inorganic nitrogen (N) and total nitrogen (TN-N) with ammonium (NH4+-N) preference. N degradation rate of NH4+-N, nitrite (NO2−-N) and nitrate (NO3−-N) were 3.9 mgL−1h−1, 3.0 mgL−1h−1 and 2.7 mgL−1h−1, respectively. In addition, strain J08 could effectively utilize most of detected low-molecular-weight carbon (LMWC) sources to degrade inorganic N with a wide adaptability to various culture conditions. Whole genome sequencing (WGS) analysis revealed that assembled genome of stain J08 possessed the crucial genes involved in dissimilatory/assimilatory NO3−-N reduction and NH4+-N assimilation. These results indicated that strain J08 could be applied to wastewater treatment in aquaculture. |
| doi_str_mv | 10.1016/j.biortech.2024.131228 |
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•Novel HN-AD bacterium, A. pittii J08, was isolated from pond sediments.•High removals of inorganic N and TN-N were analyzed in different N conditions.•Whole genome sequence and N metabolic pathways were analyzed.•Strain J08 showed a high adaptability to various culture conditions.
A novel A. pittii J08 with heterotrophic nitrification and aerobic denitrification (HN-AD) isolated from pond sediments could rapidly degrade inorganic nitrogen (N) and total nitrogen (TN-N) with ammonium (NH4+-N) preference. N degradation rate of NH4+-N, nitrite (NO2−-N) and nitrate (NO3−-N) were 3.9 mgL−1h−1, 3.0 mgL−1h−1 and 2.7 mgL−1h−1, respectively. In addition, strain J08 could effectively utilize most of detected low-molecular-weight carbon (LMWC) sources to degrade inorganic N with a wide adaptability to various culture conditions. Whole genome sequencing (WGS) analysis revealed that assembled genome of stain J08 possessed the crucial genes involved in dissimilatory/assimilatory NO3−-N reduction and NH4+-N assimilation. These results indicated that strain J08 could be applied to wastewater treatment in aquaculture.</description><identifier>ISSN: 0960-8524</identifier><identifier>ISSN: 1873-2976</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2024.131228</identifier><identifier>PMID: 39117239</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Acinetobacter ; ammonium ; aquaculture ; bacteria ; carbon ; Degradation pathway ; denitrification ; Gene expression ; genome ; genomics ; Heterotrophic nitrification and aerobic denitrification ; nitrates ; nitrification ; nitrites ; nitrogen ; Nitrogen assimilation ; nitrogen metabolism ; total nitrogen ; wastewater treatment</subject><ispartof>Bioresource technology, 2024-09, Vol.408, p.131228, Article 131228</ispartof><rights>2024 Elsevier Ltd</rights><rights>Copyright © 2024 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c1939-3509a7a8d5d38bb9589c3b75a6b90fd5e3b757a12425b8c3b4dd3284069b3303</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960852424009325$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39117239$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ou, Jie</creatorcontrib><creatorcontrib>Xie, Qing</creatorcontrib><creatorcontrib>Zhong, Zi-Rou</creatorcontrib><creatorcontrib>Wang, Fei</creatorcontrib><creatorcontrib>Huang, Ming-Zhu</creatorcontrib><creatorcontrib>Fang, Zi-Xuan</creatorcontrib><creatorcontrib>Kuang, Xu-Ying</creatorcontrib><creatorcontrib>Qin, Zi-Le</creatorcontrib><creatorcontrib>Luo, Sheng-Wei</creatorcontrib><title>Genomic analysis and metabolic characteristics provide insights into inorganic nitrogen metabolism of novel bacterium Acinetobacter pittii J08</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>[Display omitted]
•Novel HN-AD bacterium, A. pittii J08, was isolated from pond sediments.•High removals of inorganic N and TN-N were analyzed in different N conditions.•Whole genome sequence and N metabolic pathways were analyzed.•Strain J08 showed a high adaptability to various culture conditions.
A novel A. pittii J08 with heterotrophic nitrification and aerobic denitrification (HN-AD) isolated from pond sediments could rapidly degrade inorganic nitrogen (N) and total nitrogen (TN-N) with ammonium (NH4+-N) preference. N degradation rate of NH4+-N, nitrite (NO2−-N) and nitrate (NO3−-N) were 3.9 mgL−1h−1, 3.0 mgL−1h−1 and 2.7 mgL−1h−1, respectively. In addition, strain J08 could effectively utilize most of detected low-molecular-weight carbon (LMWC) sources to degrade inorganic N with a wide adaptability to various culture conditions. Whole genome sequencing (WGS) analysis revealed that assembled genome of stain J08 possessed the crucial genes involved in dissimilatory/assimilatory NO3−-N reduction and NH4+-N assimilation. These results indicated that strain J08 could be applied to wastewater treatment in aquaculture.</description><subject>Acinetobacter</subject><subject>ammonium</subject><subject>aquaculture</subject><subject>bacteria</subject><subject>carbon</subject><subject>Degradation pathway</subject><subject>denitrification</subject><subject>Gene expression</subject><subject>genome</subject><subject>genomics</subject><subject>Heterotrophic nitrification and aerobic denitrification</subject><subject>nitrates</subject><subject>nitrification</subject><subject>nitrites</subject><subject>nitrogen</subject><subject>Nitrogen assimilation</subject><subject>nitrogen metabolism</subject><subject>total nitrogen</subject><subject>wastewater treatment</subject><issn>0960-8524</issn><issn>1873-2976</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkc1uGyEUhVHVqnHSvkLEsptx-RlmYNcoatNGkbrJHvFzbWPNgAvYUl6izxysSbLNBg6X754rOAhdU7KmhA7f92sbUq7gdmtGWL-mnDImP6AVlSPvmBqHj2hF1EA6KVh_gS5L2RNCOB3ZZ3TBFW2CqxX6fwcxzcFhE830VEJpwuMZqrFpamW3M9m4CjmUGlzBh5xOwQMOsYTtrpYmampLylsTGx9DzWkL8c2izDhtcEwnmLBdnI4zvnEhQk1LAR9CrSHgeyK_oE8bMxX4-rJfocdfPx9vf3cPf-_-3N48dI4qrjouiDKjkV54Lq1VQirH7SjMYBXZeAHnw2go65mwsl313nMmezIoyznhV-jbYtue8-8Ipeo5FAfTZCKkY9GcCj5KLhh7HyWKqF70vWjosKAup1IybPQhh9nkJ02JPqem9_o1NX1OTS-ptcbrlxlHO4N_a3uNqQE_FgDan5wCZF1cgOjAhwyuap_CezOeAT0Nrkg</recordid><startdate>20240901</startdate><enddate>20240901</enddate><creator>Ou, Jie</creator><creator>Xie, Qing</creator><creator>Zhong, Zi-Rou</creator><creator>Wang, Fei</creator><creator>Huang, Ming-Zhu</creator><creator>Fang, Zi-Xuan</creator><creator>Kuang, Xu-Ying</creator><creator>Qin, Zi-Le</creator><creator>Luo, Sheng-Wei</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240901</creationdate><title>Genomic analysis and metabolic characteristics provide insights into inorganic nitrogen metabolism of novel bacterium Acinetobacter pittii J08</title><author>Ou, Jie ; Xie, Qing ; Zhong, Zi-Rou ; Wang, Fei ; Huang, Ming-Zhu ; Fang, Zi-Xuan ; Kuang, Xu-Ying ; Qin, Zi-Le ; Luo, Sheng-Wei</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1939-3509a7a8d5d38bb9589c3b75a6b90fd5e3b757a12425b8c3b4dd3284069b3303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Acinetobacter</topic><topic>ammonium</topic><topic>aquaculture</topic><topic>bacteria</topic><topic>carbon</topic><topic>Degradation pathway</topic><topic>denitrification</topic><topic>Gene expression</topic><topic>genome</topic><topic>genomics</topic><topic>Heterotrophic nitrification and aerobic denitrification</topic><topic>nitrates</topic><topic>nitrification</topic><topic>nitrites</topic><topic>nitrogen</topic><topic>Nitrogen assimilation</topic><topic>nitrogen metabolism</topic><topic>total nitrogen</topic><topic>wastewater treatment</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ou, Jie</creatorcontrib><creatorcontrib>Xie, Qing</creatorcontrib><creatorcontrib>Zhong, Zi-Rou</creatorcontrib><creatorcontrib>Wang, Fei</creatorcontrib><creatorcontrib>Huang, Ming-Zhu</creatorcontrib><creatorcontrib>Fang, Zi-Xuan</creatorcontrib><creatorcontrib>Kuang, Xu-Ying</creatorcontrib><creatorcontrib>Qin, Zi-Le</creatorcontrib><creatorcontrib>Luo, Sheng-Wei</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Bioresource technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ou, Jie</au><au>Xie, Qing</au><au>Zhong, Zi-Rou</au><au>Wang, Fei</au><au>Huang, Ming-Zhu</au><au>Fang, Zi-Xuan</au><au>Kuang, Xu-Ying</au><au>Qin, Zi-Le</au><au>Luo, Sheng-Wei</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genomic analysis and metabolic characteristics provide insights into inorganic nitrogen metabolism of novel bacterium Acinetobacter pittii J08</atitle><jtitle>Bioresource technology</jtitle><addtitle>Bioresour Technol</addtitle><date>2024-09-01</date><risdate>2024</risdate><volume>408</volume><spage>131228</spage><pages>131228-</pages><artnum>131228</artnum><issn>0960-8524</issn><issn>1873-2976</issn><eissn>1873-2976</eissn><abstract>[Display omitted]
•Novel HN-AD bacterium, A. pittii J08, was isolated from pond sediments.•High removals of inorganic N and TN-N were analyzed in different N conditions.•Whole genome sequence and N metabolic pathways were analyzed.•Strain J08 showed a high adaptability to various culture conditions.
A novel A. pittii J08 with heterotrophic nitrification and aerobic denitrification (HN-AD) isolated from pond sediments could rapidly degrade inorganic nitrogen (N) and total nitrogen (TN-N) with ammonium (NH4+-N) preference. N degradation rate of NH4+-N, nitrite (NO2−-N) and nitrate (NO3−-N) were 3.9 mgL−1h−1, 3.0 mgL−1h−1 and 2.7 mgL−1h−1, respectively. In addition, strain J08 could effectively utilize most of detected low-molecular-weight carbon (LMWC) sources to degrade inorganic N with a wide adaptability to various culture conditions. Whole genome sequencing (WGS) analysis revealed that assembled genome of stain J08 possessed the crucial genes involved in dissimilatory/assimilatory NO3−-N reduction and NH4+-N assimilation. These results indicated that strain J08 could be applied to wastewater treatment in aquaculture.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>39117239</pmid><doi>10.1016/j.biortech.2024.131228</doi></addata></record> |
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| subjects | Acinetobacter ammonium aquaculture bacteria carbon Degradation pathway denitrification Gene expression genome genomics Heterotrophic nitrification and aerobic denitrification nitrates nitrification nitrites nitrogen Nitrogen assimilation nitrogen metabolism total nitrogen wastewater treatment |
| title | Genomic analysis and metabolic characteristics provide insights into inorganic nitrogen metabolism of novel bacterium Acinetobacter pittii J08 |
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