Genomic analysis and metabolic characteristics provide insights into inorganic nitrogen metabolism of novel bacterium Acinetobacter pittii J08

[Display omitted] •Novel HN-AD bacterium, A. pittii J08, was isolated from pond sediments.•High removals of inorganic N and TN-N were analyzed in different N conditions.•Whole genome sequence and N metabolic pathways were analyzed.•Strain J08 showed a high adaptability to various culture conditions. A novel A. pittii J08 with heterotrophic nitrification and aerobic denitrification (HN-AD) isolated from pond sediments could rapidly degrade inorganic nitrogen (N) and total nitrogen (TN-N) with ammonium (NH4+-N) preference. N degradation rate of NH4+-N, nitrite (NO2−-N) and nitrate (NO3−-N) were 3.9 mgL−1h−1, 3.0 mgL−1h−1 and 2.7 mgL−1h−1, respectively. In addition, strain J08 could effectively utilize most of detected low-molecular-weight carbon (LMWC) sources to degrade inorganic N with a wide adaptability to various culture conditions. Whole genome sequencing (WGS) analysis revealed that assembled genome of stain J08 possessed the crucial genes involved in dissimilatory/assimilatory NO3−-N reduction and NH4+-N assimilation. These results indicated that strain J08 could be applied to wastewater treatment in aquaculture.