Comparison of extraction methods in vitro Plasmodium falciparum: A 1H NMR and LC-MS joined approach

Malaria is a parasitic disease that remains a global concern and the subject of many studies. Metabolomics has emerged as an approach to better comprehend complex pathogens and discover possible drug targets, thus giving new insights that can aid in the development of antimalarial therapies. However, there is no standardized method to extract metabolites from in vitro Plasmodium falciparum intraerythrocytic parasites, the stage that causes malaria. Additionally, most methods are developed with either LC-MS or NMR analysis in mind, and have rarely been evaluated with both tools. In this work, three extraction methods frequently found in the literature were reproduced and samples were analyzed through both LC-MS and 1H NMR, and evaluated in order to reveal which is the most repeatable and consistent through an array of different tools, including chemometrics, peak detection and annotation. The most reliable method in this study proved to be a double extraction with methanol and methanol/water (80:20, v/v). Metabolomic studies in the field should move towards standardization of methodologies and the use of both LC-MS and 1H NMR in order to make data more comparable between studies and facilitate the achievement of biologically interpretable information. [Display omitted] •Metabolomics is a versatile approach to antimalarial drug discovery but there is no consensus on the methodology.•Three extraction methods were performed on in vitro Plasmodium falciparum cultures and analyzed by both NMR and LC-MS.•A double extraction with methanol and methanol/water (80:20, v/v) was the most reliable method for both NMR and LC-MS.•The application of recent statistical models and joined analytical tools paves the way for methodology standardization.