Striatum-specific expression of Cre recombinase using the Gpr88 promoter in mice

We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision...

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Veröffentlicht in:	Transgenic research 2013-12, Vol.22 (6), p.1241-1247
Hauptverfasser:	Hisatsune, Chihiro, Ogawa, Naoko, Mikoshiba, Katsuhiko
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal Genetics and Genomics Animals bacterial artificial chromosomes Basal Ganglia - metabolism beta-galactosidase beta-Galactosidase - genetics beta-Galactosidase - metabolism Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biotechnology brain Brain - metabolism Brief Communication chloramphenicol acetyltransferase Chromosomes, Artificial, Bacterial - genetics Escherichia coli excision Fundamental and applied biological sciences. Psychology ganglia genes Genetic Engineering Genetic technics genetically modified organisms Integrases - genetics Integrases - metabolism Lac Operon Life Sciences Methods. Procedures. Technologies Mice Mice, Transgenic Molecular Medicine neurons Plant Genetics and Genomics Promoter Regions, Genetic Receptors, G-Protein-Coupled - genetics recombinases Transgenic animals and transgenic plants Transgenics
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Zusammenfassung:	We generated a transgenic (Tg) mouse line expressing Cre recombinase under the control of the Gpr88 promoter within a bacterial artificial chromosome clone. We crossed the established Tg mice with reporter mice (CAG-CAT-Z Tg), which express Escherichia coli lacZ in response to Cre-mediated excision of the loxP-flanked chloramphenicol acetyltransferase gene, and examined the Cre activity in the Tg mouse brains by assessing β-galactosidase activity. Cre activity was specifically detected in the caudate-putamen, nucleus accumbens, and olfactory tubercle of the Gpr88-Cre Tg mouse brain. Medium spiny neurons within the caudate-putamen exhibited Cre activity. Thus, Gpr88-Cre Tg mice could be a useful tool for analyzing the function of the basal ganglia by using Cre/loxP systems.
ISSN:	0962-8819 1573-9368
DOI:	10.1007/s11248-013-9711-x