The involvement of CaMKKI in activating AMPKα in yesso scallop Patinopecten yessoensis under high temperature stress
Calcium/calmodulin dependent protein kinase kinase (CaMKK), a highly conserved protein kinase, is involved in the downstream processes of various biological activities by phosphorylating and activating 5′-AMP-activated protein kinase (AMPK) in response to the increase of cytosolic-free calcium (Ca2+...
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| Veröffentlicht in: | Developmental and comparative immunology 2024-10, Vol.159, p.105227, Article 105227 |
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| creator | Tong, Ziling Jiang, Dongli Yang, Chuanyan Li, Yinan He, Zhaoyu Ma, Xiaoxue Wang, Lingling Song, Linsheng |
| description | Calcium/calmodulin dependent protein kinase kinase (CaMKK), a highly conserved protein kinase, is involved in the downstream processes of various biological activities by phosphorylating and activating 5′-AMP-activated protein kinase (AMPK) in response to the increase of cytosolic-free calcium (Ca2+). In the present study, a CaMKKI was identified from Yesso scallop Patinopecten yessoensis. Its mRNA was ubiquitously expressed in haemocytes and all tested tissues with the highest expression level in mantle. The expression level of PyCaMKKI mRNA in adductor muscle was significantly upregulated at 1, 3 and 6 h after high temperature treatment (25 °C), which was 3.43-fold (p |
| doi_str_mv | 10.1016/j.dci.2024.105227 |
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•PyCaMKKI with a classical S_TKc kinase domain was identified from Patinopecten yessoensis.•PyCaMKKI mRNA was ubiquitously expressed with the highest expression in mantle.•PyCaMKKI and PyAMPKα could bind each other both in vivo and in vitro.•The PyCaMKKI-PyAMPKα pathway was activated after high temperature treatment.•Knockdown PyCaMKKI inhibited the activation of PyAMPKα under high temperature stress.</description><identifier>ISSN: 0145-305X</identifier><identifier>ISSN: 1879-0089</identifier><identifier>EISSN: 1879-0089</identifier><identifier>DOI: 10.1016/j.dci.2024.105227</identifier><identifier>PMID: 38986890</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>5′-AMP-Activated protein kinase ; Calcium ; Calcium/calmodulin dependent protein kinase kinase ; calmodulin ; energy ; fluorescence ; heat stress ; heat treatment ; hemocytes ; High temperature stress ; homeostasis ; immunology ; Mizuhopecten yessoensis ; muscles ; Patinopecten yessoensis ; phosphorylation ; protein content ; protein kinases</subject><ispartof>Developmental and comparative immunology, 2024-10, Vol.159, p.105227, Article 105227</ispartof><rights>2024 Elsevier Ltd</rights><rights>Copyright © 2024. Published by Elsevier Ltd.</rights><rights>Copyright © 2024 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c268t-e476800f796d1aff124fb3123d87f94440fdaceeed1776982e4faf457aac9cf53</cites><orcidid>0000-0002-4945-1166</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0145305X24000995$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38986890$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tong, Ziling</creatorcontrib><creatorcontrib>Jiang, Dongli</creatorcontrib><creatorcontrib>Yang, Chuanyan</creatorcontrib><creatorcontrib>Li, Yinan</creatorcontrib><creatorcontrib>He, Zhaoyu</creatorcontrib><creatorcontrib>Ma, Xiaoxue</creatorcontrib><creatorcontrib>Wang, Lingling</creatorcontrib><creatorcontrib>Song, Linsheng</creatorcontrib><title>The involvement of CaMKKI in activating AMPKα in yesso scallop Patinopecten yessoensis under high temperature stress</title><title>Developmental and comparative immunology</title><addtitle>Dev Comp Immunol</addtitle><description>Calcium/calmodulin dependent protein kinase kinase (CaMKK), a highly conserved protein kinase, is involved in the downstream processes of various biological activities by phosphorylating and activating 5′-AMP-activated protein kinase (AMPK) in response to the increase of cytosolic-free calcium (Ca2+). In the present study, a CaMKKI was identified from Yesso scallop Patinopecten yessoensis. Its mRNA was ubiquitously expressed in haemocytes and all tested tissues with the highest expression level in mantle. The expression level of PyCaMKKI mRNA in adductor muscle was significantly upregulated at 1, 3 and 6 h after high temperature treatment (25 °C), which was 3.43-fold (p < 0.05), 5.25-fold (p < 0.05), and 5.70-fold (p < 0.05) of that in blank group, respectively. At 3 h after high temperature treatment (25 °C), the protein level of PyAMPKα, as well as the phosphorylation level of PyAMPKα at Thr170 in adductor muscle, and the positive co-localized fluorescence signals of PyCaMKKI and PyAMPKα in haemocyte all increased significantly (p < 0.05) compared to blank group (18 °C). The pull-down assay showed that rPyCaMKKI and rPyAMPKα could bind each other in vitro. After PyCaMKKI was silenced by siRNA, the mRNA and protein levels of PyCaMKKI and PyAMPKα, and the phosphorylation level of PyAMPKα at Thr170 in adductor muscle were significantly down-regulated (p < 0.05) compared with the negative control group receiving an injection of siRNA-NC. These results collectively suggested that PyCaMKKI was involved in the activation of PyAMPKα in response to high temperature stress and would be helpful for understanding the function of PyCaMKKI-PyAMPKα pathway in maintaining energy homeostasis under high temperature stress in scallops.
•PyCaMKKI with a classical S_TKc kinase domain was identified from Patinopecten yessoensis.•PyCaMKKI mRNA was ubiquitously expressed with the highest expression in mantle.•PyCaMKKI and PyAMPKα could bind each other both in vivo and in vitro.•The PyCaMKKI-PyAMPKα pathway was activated after high temperature treatment.•Knockdown PyCaMKKI inhibited the activation of PyAMPKα under high temperature stress.</description><subject>5′-AMP-Activated protein kinase</subject><subject>Calcium</subject><subject>Calcium/calmodulin dependent protein kinase kinase</subject><subject>calmodulin</subject><subject>energy</subject><subject>fluorescence</subject><subject>heat stress</subject><subject>heat treatment</subject><subject>hemocytes</subject><subject>High temperature stress</subject><subject>homeostasis</subject><subject>immunology</subject><subject>Mizuhopecten yessoensis</subject><subject>muscles</subject><subject>Patinopecten yessoensis</subject><subject>phosphorylation</subject><subject>protein content</subject><subject>protein kinases</subject><issn>0145-305X</issn><issn>1879-0089</issn><issn>1879-0089</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNqFkc1uEzEQxy0EoqHlAbggH7ls8NeuveJURYVWaUUPrcTNcu1x42i_sL2R-li8CM9URwkcW19GnvnN_zA_hD5RsqSENl-3S2fDkhEmyr9mTL5BC6pkWxGi2rdoQaioK07qXyfoQ0pbUp6i5D064apVjWrJAs13G8Bh2I3dDnoYMh49Xpmb9fqqdLGxOexMDsMjPr-5Xf_9s28-QUojTtZ03Tjh2_14nMBmOI5gSCHheXAQ8SY8bnCGfoJo8hwBpxwLc4beedMl-Hisp-j--8Xd6rK6_vnjanV-XVnWqFyBkI0ixMu2cdR4T5nwD5wy7pT0rRCCeGcsADgqZdMqBsIbL2ppjG2tr_kp-nLIneL4e4aUdR-Sha4zA4xz0pzWXHKmpHwdJVJJWquGF5QeUBvHlCJ4PcXQm_ikKdF7MXqrixi9F6MPYsrO52P8_NCD-7_xz0QBvh0AKPfYBYg62QCDBRdiOa52Y3gh_hluj6AY</recordid><startdate>20241001</startdate><enddate>20241001</enddate><creator>Tong, Ziling</creator><creator>Jiang, Dongli</creator><creator>Yang, Chuanyan</creator><creator>Li, Yinan</creator><creator>He, Zhaoyu</creator><creator>Ma, Xiaoxue</creator><creator>Wang, Lingling</creator><creator>Song, Linsheng</creator><general>Elsevier Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-4945-1166</orcidid></search><sort><creationdate>20241001</creationdate><title>The involvement of CaMKKI in activating AMPKα in yesso scallop Patinopecten yessoensis under high temperature stress</title><author>Tong, Ziling ; Jiang, Dongli ; Yang, Chuanyan ; Li, Yinan ; He, Zhaoyu ; Ma, Xiaoxue ; Wang, Lingling ; Song, Linsheng</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c268t-e476800f796d1aff124fb3123d87f94440fdaceeed1776982e4faf457aac9cf53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>5′-AMP-Activated protein kinase</topic><topic>Calcium</topic><topic>Calcium/calmodulin dependent protein kinase kinase</topic><topic>calmodulin</topic><topic>energy</topic><topic>fluorescence</topic><topic>heat stress</topic><topic>heat treatment</topic><topic>hemocytes</topic><topic>High temperature stress</topic><topic>homeostasis</topic><topic>immunology</topic><topic>Mizuhopecten yessoensis</topic><topic>muscles</topic><topic>Patinopecten yessoensis</topic><topic>phosphorylation</topic><topic>protein content</topic><topic>protein kinases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tong, Ziling</creatorcontrib><creatorcontrib>Jiang, Dongli</creatorcontrib><creatorcontrib>Yang, Chuanyan</creatorcontrib><creatorcontrib>Li, Yinan</creatorcontrib><creatorcontrib>He, Zhaoyu</creatorcontrib><creatorcontrib>Ma, Xiaoxue</creatorcontrib><creatorcontrib>Wang, Lingling</creatorcontrib><creatorcontrib>Song, Linsheng</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Developmental and comparative immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tong, Ziling</au><au>Jiang, Dongli</au><au>Yang, Chuanyan</au><au>Li, Yinan</au><au>He, Zhaoyu</au><au>Ma, Xiaoxue</au><au>Wang, Lingling</au><au>Song, Linsheng</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The involvement of CaMKKI in activating AMPKα in yesso scallop Patinopecten yessoensis under high temperature stress</atitle><jtitle>Developmental and comparative immunology</jtitle><addtitle>Dev Comp Immunol</addtitle><date>2024-10-01</date><risdate>2024</risdate><volume>159</volume><spage>105227</spage><pages>105227-</pages><artnum>105227</artnum><issn>0145-305X</issn><issn>1879-0089</issn><eissn>1879-0089</eissn><abstract>Calcium/calmodulin dependent protein kinase kinase (CaMKK), a highly conserved protein kinase, is involved in the downstream processes of various biological activities by phosphorylating and activating 5′-AMP-activated protein kinase (AMPK) in response to the increase of cytosolic-free calcium (Ca2+). In the present study, a CaMKKI was identified from Yesso scallop Patinopecten yessoensis. Its mRNA was ubiquitously expressed in haemocytes and all tested tissues with the highest expression level in mantle. The expression level of PyCaMKKI mRNA in adductor muscle was significantly upregulated at 1, 3 and 6 h after high temperature treatment (25 °C), which was 3.43-fold (p < 0.05), 5.25-fold (p < 0.05), and 5.70-fold (p < 0.05) of that in blank group, respectively. At 3 h after high temperature treatment (25 °C), the protein level of PyAMPKα, as well as the phosphorylation level of PyAMPKα at Thr170 in adductor muscle, and the positive co-localized fluorescence signals of PyCaMKKI and PyAMPKα in haemocyte all increased significantly (p < 0.05) compared to blank group (18 °C). The pull-down assay showed that rPyCaMKKI and rPyAMPKα could bind each other in vitro. After PyCaMKKI was silenced by siRNA, the mRNA and protein levels of PyCaMKKI and PyAMPKα, and the phosphorylation level of PyAMPKα at Thr170 in adductor muscle were significantly down-regulated (p < 0.05) compared with the negative control group receiving an injection of siRNA-NC. These results collectively suggested that PyCaMKKI was involved in the activation of PyAMPKα in response to high temperature stress and would be helpful for understanding the function of PyCaMKKI-PyAMPKα pathway in maintaining energy homeostasis under high temperature stress in scallops.
•PyCaMKKI with a classical S_TKc kinase domain was identified from Patinopecten yessoensis.•PyCaMKKI mRNA was ubiquitously expressed with the highest expression in mantle.•PyCaMKKI and PyAMPKα could bind each other both in vivo and in vitro.•The PyCaMKKI-PyAMPKα pathway was activated after high temperature treatment.•Knockdown PyCaMKKI inhibited the activation of PyAMPKα under high temperature stress.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>38986890</pmid><doi>10.1016/j.dci.2024.105227</doi><orcidid>https://orcid.org/0000-0002-4945-1166</orcidid></addata></record> |
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| subjects | 5′-AMP-Activated protein kinase Calcium Calcium/calmodulin dependent protein kinase kinase calmodulin energy fluorescence heat stress heat treatment hemocytes High temperature stress homeostasis immunology Mizuhopecten yessoensis muscles Patinopecten yessoensis phosphorylation protein content protein kinases |
| title | The involvement of CaMKKI in activating AMPKα in yesso scallop Patinopecten yessoensis under high temperature stress |
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