Immunohistochemical and transcriptomic characterization of T and myeloid cell infiltrates in canine malignant melanoma
Immune checkpoint inhibitor therapy can provide significant clinical benefit in patients with certain cancer types including melanoma; however, objective responses are only observed for a subset of patients. Mucosal melanoma is a rare melanoma subtype associated with a poor prognosis and, compared w...
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Veröffentlicht in: | Veterinary & comparative oncology 2024-09, Vol.22 (3), p.377-387 |
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description | Immune checkpoint inhibitor therapy can provide significant clinical benefit in patients with certain cancer types including melanoma; however, objective responses are only observed for a subset of patients. Mucosal melanoma is a rare melanoma subtype associated with a poor prognosis and, compared with cutaneous melanoma, is significantly less responsive to immune checkpoint inhibitors. Spontaneous canine tumours have emerged as valuable models to inform human cancer studies. In contrast to human melanoma, most canine melanomas are mucosal—an incidence that may be leveraged to better understand the subtype in humans. However, a more comprehensive understanding of the immune landscape of the canine disease is required. Here, we quantify tumour infiltrative T and myeloid cells in canine mucosal (n = 13) and cutaneous (n = 5) melanomas using immunohistochemical analysis of CD3 and MAC387 expression, respectively. Gene expression analysis using the Canine IO NanoString panel was also performed to identify genes and pathways associated with immune cell infiltration. T and myeloid cell densities were variable with geometric means of 158.7 cells/mm2 and 166.7 cells/mm2, respectively. Elevated T cell infiltration was associated with increased expression of cytolytic genes as well as genes encoding the coinhibitory checkpoint molecules PD‐1, CTLA‐4, TIM‐3 and TIGIT; whereas increased myeloid cell infiltration was associated with elevated expression of protumourigenic cytokines. These data provide a basic characterization of the tumour microenvironment of canine malignant melanoma and suggest that, like human melanoma, inherent variability in anti‐tumour T cell responses exists and that a subset of canine melanomas may respond better to immunomodulation. |
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Mucosal melanoma is a rare melanoma subtype associated with a poor prognosis and, compared with cutaneous melanoma, is significantly less responsive to immune checkpoint inhibitors. Spontaneous canine tumours have emerged as valuable models to inform human cancer studies. In contrast to human melanoma, most canine melanomas are mucosal—an incidence that may be leveraged to better understand the subtype in humans. However, a more comprehensive understanding of the immune landscape of the canine disease is required. Here, we quantify tumour infiltrative T and myeloid cells in canine mucosal (n = 13) and cutaneous (n = 5) melanomas using immunohistochemical analysis of CD3 and MAC387 expression, respectively. Gene expression analysis using the Canine IO NanoString panel was also performed to identify genes and pathways associated with immune cell infiltration. T and myeloid cell densities were variable with geometric means of 158.7 cells/mm2 and 166.7 cells/mm2, respectively. Elevated T cell infiltration was associated with increased expression of cytolytic genes as well as genes encoding the coinhibitory checkpoint molecules PD‐1, CTLA‐4, TIM‐3 and TIGIT; whereas increased myeloid cell infiltration was associated with elevated expression of protumourigenic cytokines. These data provide a basic characterization of the tumour microenvironment of canine malignant melanoma and suggest that, like human melanoma, inherent variability in anti‐tumour T cell responses exists and that a subset of canine melanomas may respond better to immunomodulation.</description><identifier>ISSN: 1476-5810</identifier><identifier>ISSN: 1476-5829</identifier><identifier>EISSN: 1476-5829</identifier><identifier>DOI: 10.1111/vco.12981</identifier><identifier>PMID: 38752589</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>canine ; CTLA‐4 ; cytokines ; dog diseases ; dogs ; gene expression ; geometry ; humans ; immunohistochemistry ; immunomodulation ; immunotherapy ; melanoma ; mucosal melanoma ; PD‐1 ; prognosis ; T-lymphocytes ; therapeutics ; transcriptomics</subject><ispartof>Veterinary & comparative oncology, 2024-09, Vol.22 (3), p.377-387</ispartof><rights>2024 The Authors. published by John Wiley & Sons Ltd.</rights><rights>2024 The Authors. 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Mucosal melanoma is a rare melanoma subtype associated with a poor prognosis and, compared with cutaneous melanoma, is significantly less responsive to immune checkpoint inhibitors. Spontaneous canine tumours have emerged as valuable models to inform human cancer studies. In contrast to human melanoma, most canine melanomas are mucosal—an incidence that may be leveraged to better understand the subtype in humans. However, a more comprehensive understanding of the immune landscape of the canine disease is required. Here, we quantify tumour infiltrative T and myeloid cells in canine mucosal (n = 13) and cutaneous (n = 5) melanomas using immunohistochemical analysis of CD3 and MAC387 expression, respectively. Gene expression analysis using the Canine IO NanoString panel was also performed to identify genes and pathways associated with immune cell infiltration. T and myeloid cell densities were variable with geometric means of 158.7 cells/mm2 and 166.7 cells/mm2, respectively. Elevated T cell infiltration was associated with increased expression of cytolytic genes as well as genes encoding the coinhibitory checkpoint molecules PD‐1, CTLA‐4, TIM‐3 and TIGIT; whereas increased myeloid cell infiltration was associated with elevated expression of protumourigenic cytokines. These data provide a basic characterization of the tumour microenvironment of canine malignant melanoma and suggest that, like human melanoma, inherent variability in anti‐tumour T cell responses exists and that a subset of canine melanomas may respond better to immunomodulation.</description><subject>canine</subject><subject>CTLA‐4</subject><subject>cytokines</subject><subject>dog diseases</subject><subject>dogs</subject><subject>gene expression</subject><subject>geometry</subject><subject>humans</subject><subject>immunohistochemistry</subject><subject>immunomodulation</subject><subject>immunotherapy</subject><subject>melanoma</subject><subject>mucosal melanoma</subject><subject>PD‐1</subject><subject>prognosis</subject><subject>T-lymphocytes</subject><subject>therapeutics</subject><subject>transcriptomics</subject><issn>1476-5810</issn><issn>1476-5829</issn><issn>1476-5829</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>24P</sourceid><recordid>eNqFkUlPAzEMhSMEgrIc-AMoRzgUsjTt5IgqNgmJC3AdeTIeGpSlJNOi8usJLXBD-GLL-t6T5UfIMWfnvNTF0sRzLnTFt8iAjybjoaqE3v6dOdsj-zm_MibESIpdsieriRKq0gOyvPN-EeLM5j6aGXprwFEILe0ThGySnfexLKmZQQLTY7If0NsYaOzo4xr0K3TRttSgc9SGzroi7TGXmRoINiD14OxLgNBTjw5C9HBIdjpwGY---wF5ur56nN4O7x9u7qaX90MjecWHWgvFdGXaMTZGNEyqtsFKIyrDOG9bIbQ2nDWqg46DEAisNbwTk0aookV5QE43vvMU3xaY-9rb_HUpBIyLXEuu5FhrySf_o0yVj0kxGhX0bIOaFHNO2NXzZD2kVc1Z_ZVIXRKp14kU9uTbdtF4bH_JnwgKcLEB3q3D1d9O9fP0YWP5CeOxl7c</recordid><startdate>202409</startdate><enddate>202409</enddate><creator>Cronise, Kathryn E.</creator><creator>Coy, Jonathan</creator><creator>Dow, Steven</creator><creator>Hauck, Marlene L.</creator><creator>Regan, Daniel P.</creator><general>Blackwell Publishing Ltd</general><scope>24P</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope><orcidid>https://orcid.org/0000-0002-3148-1332</orcidid></search><sort><creationdate>202409</creationdate><title>Immunohistochemical and transcriptomic characterization of T and myeloid cell infiltrates in canine malignant melanoma</title><author>Cronise, Kathryn E. ; Coy, Jonathan ; Dow, Steven ; Hauck, Marlene L. ; Regan, Daniel P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3181-9925098cd6ebc2b035dbe89ee5c011dd2299c10b5faf1a22ea0dc1f27b25992e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>canine</topic><topic>CTLA‐4</topic><topic>cytokines</topic><topic>dog diseases</topic><topic>dogs</topic><topic>gene expression</topic><topic>geometry</topic><topic>humans</topic><topic>immunohistochemistry</topic><topic>immunomodulation</topic><topic>immunotherapy</topic><topic>melanoma</topic><topic>mucosal melanoma</topic><topic>PD‐1</topic><topic>prognosis</topic><topic>T-lymphocytes</topic><topic>therapeutics</topic><topic>transcriptomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Cronise, Kathryn E.</creatorcontrib><creatorcontrib>Coy, Jonathan</creatorcontrib><creatorcontrib>Dow, Steven</creatorcontrib><creatorcontrib>Hauck, Marlene L.</creatorcontrib><creatorcontrib>Regan, Daniel P.</creatorcontrib><collection>Wiley Online Library Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Veterinary & comparative oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cronise, Kathryn E.</au><au>Coy, Jonathan</au><au>Dow, Steven</au><au>Hauck, Marlene L.</au><au>Regan, Daniel P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical and transcriptomic characterization of T and myeloid cell infiltrates in canine malignant melanoma</atitle><jtitle>Veterinary & comparative oncology</jtitle><addtitle>Vet Comp Oncol</addtitle><date>2024-09</date><risdate>2024</risdate><volume>22</volume><issue>3</issue><spage>377</spage><epage>387</epage><pages>377-387</pages><issn>1476-5810</issn><issn>1476-5829</issn><eissn>1476-5829</eissn><abstract>Immune checkpoint inhibitor therapy can provide significant clinical benefit in patients with certain cancer types including melanoma; however, objective responses are only observed for a subset of patients. Mucosal melanoma is a rare melanoma subtype associated with a poor prognosis and, compared with cutaneous melanoma, is significantly less responsive to immune checkpoint inhibitors. Spontaneous canine tumours have emerged as valuable models to inform human cancer studies. In contrast to human melanoma, most canine melanomas are mucosal—an incidence that may be leveraged to better understand the subtype in humans. However, a more comprehensive understanding of the immune landscape of the canine disease is required. Here, we quantify tumour infiltrative T and myeloid cells in canine mucosal (n = 13) and cutaneous (n = 5) melanomas using immunohistochemical analysis of CD3 and MAC387 expression, respectively. Gene expression analysis using the Canine IO NanoString panel was also performed to identify genes and pathways associated with immune cell infiltration. T and myeloid cell densities were variable with geometric means of 158.7 cells/mm2 and 166.7 cells/mm2, respectively. Elevated T cell infiltration was associated with increased expression of cytolytic genes as well as genes encoding the coinhibitory checkpoint molecules PD‐1, CTLA‐4, TIM‐3 and TIGIT; whereas increased myeloid cell infiltration was associated with elevated expression of protumourigenic cytokines. These data provide a basic characterization of the tumour microenvironment of canine malignant melanoma and suggest that, like human melanoma, inherent variability in anti‐tumour T cell responses exists and that a subset of canine melanomas may respond better to immunomodulation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>38752589</pmid><doi>10.1111/vco.12981</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-3148-1332</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | canine CTLA‐4 cytokines dog diseases dogs gene expression geometry humans immunohistochemistry immunomodulation immunotherapy melanoma mucosal melanoma PD‐1 prognosis T-lymphocytes therapeutics transcriptomics |
title | Immunohistochemical and transcriptomic characterization of T and myeloid cell infiltrates in canine malignant melanoma |
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