Recent Advances in Rapid Screening Methods for Glycosyltransferases

Glycosyltransferases (GTs) are a diverse group of enzymes widely distributed in nature that catalyze the transfer of glycosyl moieties from activated sugar donors to a diverse range of acceptor molecules. GTs play important roles in various biological processes, such as cellular signal transduction, cell–cell interactions, immune response and disease. In addition, the utilization of glycosyltransferases to synthesize glycosides has become a major area of pharmaceutical research. The construction of a rapid and efficient screening method for glycosyltransferases is the key to the successful discovery, identification, and modification of new enzymes. In the past few decades, a series of screening methods have been developed to assess GT activity. This review focuses on various methods for rapid screening of GTs, outlining their advantages and disadvantages. Graphical Abstract