Nesterenkonia marinintestina sp. nov., isolated from the fish intestine

A Gram-positive, aerobic, non-motile, irregular short rod, nonspore-forming actinobacterial strain, designated GX14115 T , was isolated from fish intestine in Beihai City, Guangxi, China and subjected to a taxonomic polyphasic investigation. Colonies were yellow‒green, circular, smooth, central bulg...

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Veröffentlicht in:Archives of microbiology 2024-03, Vol.206 (3), p.110-110, Article 110
Hauptverfasser: Zhao, Aolin, Cai, Hanqin, Huang, Ying, Yang, Quan, Zhu, Zhenxin, Zhou, Yan, Jiang, Mingguo, Jiang, Yi, Huang, Wenshan
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container_issue 3
container_start_page 110
container_title Archives of microbiology
container_volume 206
creator Zhao, Aolin
Cai, Hanqin
Huang, Ying
Yang, Quan
Zhu, Zhenxin
Zhou, Yan
Jiang, Mingguo
Jiang, Yi
Huang, Wenshan
description A Gram-positive, aerobic, non-motile, irregular short rod, nonspore-forming actinobacterial strain, designated GX14115 T , was isolated from fish intestine in Beihai City, Guangxi, China and subjected to a taxonomic polyphasic investigation. Colonies were yellow‒green, circular, smooth, central bulge, convex, opaque and 2.0–3.0 mm in diameter after growth on 2216E medium at 30 °C for 72 h. Growth occurred at 4–45 °C (optimum 30 °C), at pH 4.5–10.0 (optimum pH 7.5) and in the presence of 0–12% NaCl (w/v) (optimum 3.5%). Chemotaxonomic analysis showed that the main menaquinone of strain GX14115 T was MK-7. The major cellular fatty acids were anteiso -C 15:0 (44.8%), anteiso -C 17:0 (20.5%), and iso -C 15:0 (16%). The whole-cell sugars were galactose and xylose. The peptidoglycan type was l -Lys-Gly- d -Asp, and the polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), one unknown phospholipid (UP), and one unknown glycolipid (UG). The DNA G + C content of the type of strain was 69.5 mol%. The 16S rRNA gene sequence analysis revealed that strain GX14115 T is affiliated with the genus Nesterenkonia and is closely related to Nesterenkonia sandarakina YIM 70009 T (96.5%) and Nesterenkonia lutea YIM 70081 T (96.8%). The calculated results indicated that the average nucleotide identity (ANI) values of GX14115 T were 74.49–74.78%, to the two aforementioned type strains, and the digital DNA–DNA hybridization (dDDH) values were 20.1–20.7%. Strain GX14115 T was proposed as a novel species of the genus Nesterenkonia by the physiological, chemotaxonomic, and phylogenetic data, for whose the name is Nesterenkonia marinintestina sp. nov. The type of strain is GX14115 T (= MCCC 1K06658 T  = KCTC 49495 T ).
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Colonies were yellow‒green, circular, smooth, central bulge, convex, opaque and 2.0–3.0 mm in diameter after growth on 2216E medium at 30 °C for 72 h. Growth occurred at 4–45 °C (optimum 30 °C), at pH 4.5–10.0 (optimum pH 7.5) and in the presence of 0–12% NaCl (w/v) (optimum 3.5%). Chemotaxonomic analysis showed that the main menaquinone of strain GX14115 T was MK-7. The major cellular fatty acids were anteiso -C 15:0 (44.8%), anteiso -C 17:0 (20.5%), and iso -C 15:0 (16%). The whole-cell sugars were galactose and xylose. The peptidoglycan type was l -Lys-Gly- d -Asp, and the polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), one unknown phospholipid (UP), and one unknown glycolipid (UG). The DNA G + C content of the type of strain was 69.5 mol%. The 16S rRNA gene sequence analysis revealed that strain GX14115 T is affiliated with the genus Nesterenkonia and is closely related to Nesterenkonia sandarakina YIM 70009 T (96.5%) and Nesterenkonia lutea YIM 70081 T (96.8%). The calculated results indicated that the average nucleotide identity (ANI) values of GX14115 T were 74.49–74.78%, to the two aforementioned type strains, and the digital DNA–DNA hybridization (dDDH) values were 20.1–20.7%. Strain GX14115 T was proposed as a novel species of the genus Nesterenkonia by the physiological, chemotaxonomic, and phylogenetic data, for whose the name is Nesterenkonia marinintestina sp. nov. 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Colonies were yellow‒green, circular, smooth, central bulge, convex, opaque and 2.0–3.0 mm in diameter after growth on 2216E medium at 30 °C for 72 h. Growth occurred at 4–45 °C (optimum 30 °C), at pH 4.5–10.0 (optimum pH 7.5) and in the presence of 0–12% NaCl (w/v) (optimum 3.5%). Chemotaxonomic analysis showed that the main menaquinone of strain GX14115 T was MK-7. The major cellular fatty acids were anteiso -C 15:0 (44.8%), anteiso -C 17:0 (20.5%), and iso -C 15:0 (16%). The whole-cell sugars were galactose and xylose. The peptidoglycan type was l -Lys-Gly- d -Asp, and the polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), one unknown phospholipid (UP), and one unknown glycolipid (UG). The DNA G + C content of the type of strain was 69.5 mol%. The 16S rRNA gene sequence analysis revealed that strain GX14115 T is affiliated with the genus Nesterenkonia and is closely related to Nesterenkonia sandarakina YIM 70009 T (96.5%) and Nesterenkonia lutea YIM 70081 T (96.8%). The calculated results indicated that the average nucleotide identity (ANI) values of GX14115 T were 74.49–74.78%, to the two aforementioned type strains, and the digital DNA–DNA hybridization (dDDH) values were 20.1–20.7%. Strain GX14115 T was proposed as a novel species of the genus Nesterenkonia by the physiological, chemotaxonomic, and phylogenetic data, for whose the name is Nesterenkonia marinintestina sp. nov. 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Colonies were yellow‒green, circular, smooth, central bulge, convex, opaque and 2.0–3.0 mm in diameter after growth on 2216E medium at 30 °C for 72 h. Growth occurred at 4–45 °C (optimum 30 °C), at pH 4.5–10.0 (optimum pH 7.5) and in the presence of 0–12% NaCl (w/v) (optimum 3.5%). Chemotaxonomic analysis showed that the main menaquinone of strain GX14115 T was MK-7. The major cellular fatty acids were anteiso -C 15:0 (44.8%), anteiso -C 17:0 (20.5%), and iso -C 15:0 (16%). The whole-cell sugars were galactose and xylose. The peptidoglycan type was l -Lys-Gly- d -Asp, and the polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), one unknown phospholipid (UP), and one unknown glycolipid (UG). The DNA G + C content of the type of strain was 69.5 mol%. The 16S rRNA gene sequence analysis revealed that strain GX14115 T is affiliated with the genus Nesterenkonia and is closely related to Nesterenkonia sandarakina YIM 70009 T (96.5%) and Nesterenkonia lutea YIM 70081 T (96.8%). The calculated results indicated that the average nucleotide identity (ANI) values of GX14115 T were 74.49–74.78%, to the two aforementioned type strains, and the digital DNA–DNA hybridization (dDDH) values were 20.1–20.7%. Strain GX14115 T was proposed as a novel species of the genus Nesterenkonia by the physiological, chemotaxonomic, and phylogenetic data, for whose the name is Nesterenkonia marinintestina sp. nov. The type of strain is GX14115 T (= MCCC 1K06658 T  = KCTC 49495 T ).</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38369673</pmid><doi>10.1007/s00203-023-03825-0</doi><tpages>1</tpages></addata></record>
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source MEDLINE; SpringerLink Journals - AutoHoldings
subjects Antibiotics
Archives & records
Bacterial Typing Techniques
Biochemistry
Biomedical and Life Sciences
Biotechnology
Cardiolipin
Cell Biology
chemotaxonomy
China
Deoxyribonucleic acid
DNA
DNA, Bacterial - genetics
Ecology
Fatty Acids - analysis
Fish
Galactose
Genomes
genus
glycolipids
Hybridization
Intestine
intestines
Life Sciences
Lipids
Menaquinones
Microbial Ecology
Microbiology
Microscopy
Nesterenkonia
New species
nucleic acid hybridization
nucleotide sequences
Nucleotides
Original Paper
Peptidoglycans
Phosphatidylethanolamine
phosphatidylethanolamines
Phospholipids
Phospholipids - analysis
Phylogenetics
Phylogeny
Physiology
RNA, Ribosomal, 16S - genetics
rRNA 16S
Seawater
Sequence analysis
Sequence Analysis, DNA
Sodium chloride
species
Taxonomy
xylose
title Nesterenkonia marinintestina sp. nov., isolated from the fish intestine
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