Nesterenkonia marinintestina sp. nov., isolated from the fish intestine
A Gram-positive, aerobic, non-motile, irregular short rod, nonspore-forming actinobacterial strain, designated GX14115 T , was isolated from fish intestine in Beihai City, Guangxi, China and subjected to a taxonomic polyphasic investigation. Colonies were yellow‒green, circular, smooth, central bulg...
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description | A Gram-positive, aerobic, non-motile, irregular short rod, nonspore-forming actinobacterial strain, designated GX14115
T
, was isolated from fish intestine in Beihai City, Guangxi, China and subjected to a taxonomic polyphasic investigation. Colonies were yellow‒green, circular, smooth, central bulge, convex, opaque and 2.0–3.0 mm in diameter after growth on 2216E medium at 30 °C for 72 h. Growth occurred at 4–45 °C (optimum 30 °C), at pH 4.5–10.0 (optimum pH 7.5) and in the presence of 0–12% NaCl (w/v) (optimum 3.5%). Chemotaxonomic analysis showed that the main menaquinone of strain GX14115
T
was MK-7. The major cellular fatty acids were
anteiso
-C
15:0
(44.8%),
anteiso
-C
17:0
(20.5%), and
iso
-C
15:0
(16%). The whole-cell sugars were galactose and xylose. The peptidoglycan type was
l
-Lys-Gly-
d
-Asp, and the polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), one unknown phospholipid (UP), and one unknown glycolipid (UG). The DNA G + C content of the type of strain was 69.5 mol%. The 16S rRNA gene sequence analysis revealed that strain GX14115
T
is affiliated with the genus
Nesterenkonia
and is closely related to
Nesterenkonia sandarakina
YIM 70009
T
(96.5%) and
Nesterenkonia lutea
YIM 70081
T
(96.8%). The calculated results indicated that the average nucleotide identity (ANI) values of GX14115
T
were 74.49–74.78%, to the two aforementioned type strains, and the digital DNA–DNA hybridization (dDDH) values were 20.1–20.7%. Strain GX14115
T
was proposed as a novel species of the genus
Nesterenkonia
by the physiological, chemotaxonomic, and phylogenetic data, for whose the name is
Nesterenkonia marinintestina
sp. nov. The type of strain is GX14115
T
(= MCCC 1K06658
T
= KCTC 49495
T
). |
doi_str_mv | 10.1007/s00203-023-03825-0 |
format | Article |
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T
, was isolated from fish intestine in Beihai City, Guangxi, China and subjected to a taxonomic polyphasic investigation. Colonies were yellow‒green, circular, smooth, central bulge, convex, opaque and 2.0–3.0 mm in diameter after growth on 2216E medium at 30 °C for 72 h. Growth occurred at 4–45 °C (optimum 30 °C), at pH 4.5–10.0 (optimum pH 7.5) and in the presence of 0–12% NaCl (w/v) (optimum 3.5%). Chemotaxonomic analysis showed that the main menaquinone of strain GX14115
T
was MK-7. The major cellular fatty acids were
anteiso
-C
15:0
(44.8%),
anteiso
-C
17:0
(20.5%), and
iso
-C
15:0
(16%). The whole-cell sugars were galactose and xylose. The peptidoglycan type was
l
-Lys-Gly-
d
-Asp, and the polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), one unknown phospholipid (UP), and one unknown glycolipid (UG). The DNA G + C content of the type of strain was 69.5 mol%. The 16S rRNA gene sequence analysis revealed that strain GX14115
T
is affiliated with the genus
Nesterenkonia
and is closely related to
Nesterenkonia sandarakina
YIM 70009
T
(96.5%) and
Nesterenkonia lutea
YIM 70081
T
(96.8%). The calculated results indicated that the average nucleotide identity (ANI) values of GX14115
T
were 74.49–74.78%, to the two aforementioned type strains, and the digital DNA–DNA hybridization (dDDH) values were 20.1–20.7%. Strain GX14115
T
was proposed as a novel species of the genus
Nesterenkonia
by the physiological, chemotaxonomic, and phylogenetic data, for whose the name is
Nesterenkonia marinintestina
sp. nov. The type of strain is GX14115
T
(= MCCC 1K06658
T
= KCTC 49495
T
).</description><identifier>ISSN: 0302-8933</identifier><identifier>EISSN: 1432-072X</identifier><identifier>DOI: 10.1007/s00203-023-03825-0</identifier><identifier>PMID: 38369673</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Antibiotics ; Archives & records ; Bacterial Typing Techniques ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cardiolipin ; Cell Biology ; chemotaxonomy ; China ; Deoxyribonucleic acid ; DNA ; DNA, Bacterial - genetics ; Ecology ; Fatty Acids - analysis ; Fish ; Galactose ; Genomes ; genus ; glycolipids ; Hybridization ; Intestine ; intestines ; Life Sciences ; Lipids ; Menaquinones ; Microbial Ecology ; Microbiology ; Microscopy ; Nesterenkonia ; New species ; nucleic acid hybridization ; nucleotide sequences ; Nucleotides ; Original Paper ; Peptidoglycans ; Phosphatidylethanolamine ; phosphatidylethanolamines ; Phospholipids ; Phospholipids - analysis ; Phylogenetics ; Phylogeny ; Physiology ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Seawater ; Sequence analysis ; Sequence Analysis, DNA ; Sodium chloride ; species ; Taxonomy ; xylose</subject><ispartof>Archives of microbiology, 2024-03, Vol.206 (3), p.110-110, Article 110</ispartof><rights>The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.</rights><rights>2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c359t-628906c52e81d1f30899921b14a8cc95c84993363aa918c4ecc106c62e312ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00203-023-03825-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00203-023-03825-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38369673$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Aolin</creatorcontrib><creatorcontrib>Cai, Hanqin</creatorcontrib><creatorcontrib>Huang, Ying</creatorcontrib><creatorcontrib>Yang, Quan</creatorcontrib><creatorcontrib>Zhu, Zhenxin</creatorcontrib><creatorcontrib>Zhou, Yan</creatorcontrib><creatorcontrib>Jiang, Mingguo</creatorcontrib><creatorcontrib>Jiang, Yi</creatorcontrib><creatorcontrib>Huang, Wenshan</creatorcontrib><title>Nesterenkonia marinintestina sp. nov., isolated from the fish intestine</title><title>Archives of microbiology</title><addtitle>Arch Microbiol</addtitle><addtitle>Arch Microbiol</addtitle><description>A Gram-positive, aerobic, non-motile, irregular short rod, nonspore-forming actinobacterial strain, designated GX14115
T
, was isolated from fish intestine in Beihai City, Guangxi, China and subjected to a taxonomic polyphasic investigation. Colonies were yellow‒green, circular, smooth, central bulge, convex, opaque and 2.0–3.0 mm in diameter after growth on 2216E medium at 30 °C for 72 h. Growth occurred at 4–45 °C (optimum 30 °C), at pH 4.5–10.0 (optimum pH 7.5) and in the presence of 0–12% NaCl (w/v) (optimum 3.5%). Chemotaxonomic analysis showed that the main menaquinone of strain GX14115
T
was MK-7. The major cellular fatty acids were
anteiso
-C
15:0
(44.8%),
anteiso
-C
17:0
(20.5%), and
iso
-C
15:0
(16%). The whole-cell sugars were galactose and xylose. The peptidoglycan type was
l
-Lys-Gly-
d
-Asp, and the polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), one unknown phospholipid (UP), and one unknown glycolipid (UG). The DNA G + C content of the type of strain was 69.5 mol%. The 16S rRNA gene sequence analysis revealed that strain GX14115
T
is affiliated with the genus
Nesterenkonia
and is closely related to
Nesterenkonia sandarakina
YIM 70009
T
(96.5%) and
Nesterenkonia lutea
YIM 70081
T
(96.8%). The calculated results indicated that the average nucleotide identity (ANI) values of GX14115
T
were 74.49–74.78%, to the two aforementioned type strains, and the digital DNA–DNA hybridization (dDDH) values were 20.1–20.7%. Strain GX14115
T
was proposed as a novel species of the genus
Nesterenkonia
by the physiological, chemotaxonomic, and phylogenetic data, for whose the name is
Nesterenkonia marinintestina
sp. nov. The type of strain is GX14115
T
(= MCCC 1K06658
T
= KCTC 49495
T
).</description><subject>Antibiotics</subject><subject>Archives & records</subject><subject>Bacterial Typing Techniques</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Cardiolipin</subject><subject>Cell Biology</subject><subject>chemotaxonomy</subject><subject>China</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA, Bacterial - genetics</subject><subject>Ecology</subject><subject>Fatty Acids - analysis</subject><subject>Fish</subject><subject>Galactose</subject><subject>Genomes</subject><subject>genus</subject><subject>glycolipids</subject><subject>Hybridization</subject><subject>Intestine</subject><subject>intestines</subject><subject>Life Sciences</subject><subject>Lipids</subject><subject>Menaquinones</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Microscopy</subject><subject>Nesterenkonia</subject><subject>New species</subject><subject>nucleic acid hybridization</subject><subject>nucleotide sequences</subject><subject>Nucleotides</subject><subject>Original Paper</subject><subject>Peptidoglycans</subject><subject>Phosphatidylethanolamine</subject><subject>phosphatidylethanolamines</subject><subject>Phospholipids</subject><subject>Phospholipids - analysis</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Physiology</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Seawater</subject><subject>Sequence analysis</subject><subject>Sequence Analysis, DNA</subject><subject>Sodium chloride</subject><subject>species</subject><subject>Taxonomy</subject><subject>xylose</subject><issn>0302-8933</issn><issn>1432-072X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFPwyAUxonRuDn9BzyYJl482PmAQuFoFp0mi1528NYwSl1nSye0Jv73snXTxIMegOTxe98H70PoHMMYA6Q3HoAAjYGERQVhMRygIU4oiSElL4doCBRILCSlA3Ti_QoAEyHEMRpQQbnkKR2i6ZPxrXHGvjW2VFGtXGlL24ZiaVXk1-PINh_j66j0TaVak0eFa-qoXZqoKP0y2qPmFB0VqvLmbHeO0Pz-bj55iGfP08fJ7SzWlMk25kRI4JoRI3COCwpCSknwAidKaC2ZFokM7-VUKYmFTozWOPCcGIqJyekIXfWya9e8d8E6q0uvTVUpa5rOZxSz4EM45_-iRBLBBGPJBr38ha6aztnwjy0FPGVJGijSU9o13jtTZGtXhoF9ZhiyTSBZH0gWAsm2gYR9hC520t2iNvl3yz6BANAe8OHKvhr34_2H7BefNJOo</recordid><startdate>20240301</startdate><enddate>20240301</enddate><creator>Zhao, Aolin</creator><creator>Cai, Hanqin</creator><creator>Huang, Ying</creator><creator>Yang, Quan</creator><creator>Zhu, Zhenxin</creator><creator>Zhou, Yan</creator><creator>Jiang, Mingguo</creator><creator>Jiang, Yi</creator><creator>Huang, Wenshan</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>7S9</scope><scope>L.6</scope></search><sort><creationdate>20240301</creationdate><title>Nesterenkonia marinintestina sp. nov., isolated from the fish intestine</title><author>Zhao, Aolin ; Cai, Hanqin ; Huang, Ying ; Yang, Quan ; Zhu, Zhenxin ; Zhou, Yan ; Jiang, Mingguo ; Jiang, Yi ; Huang, Wenshan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-628906c52e81d1f30899921b14a8cc95c84993363aa918c4ecc106c62e312ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>Antibiotics</topic><topic>Archives & records</topic><topic>Bacterial Typing Techniques</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Cardiolipin</topic><topic>Cell Biology</topic><topic>chemotaxonomy</topic><topic>China</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA, Bacterial - genetics</topic><topic>Ecology</topic><topic>Fatty Acids - analysis</topic><topic>Fish</topic><topic>Galactose</topic><topic>Genomes</topic><topic>genus</topic><topic>glycolipids</topic><topic>Hybridization</topic><topic>Intestine</topic><topic>intestines</topic><topic>Life Sciences</topic><topic>Lipids</topic><topic>Menaquinones</topic><topic>Microbial Ecology</topic><topic>Microbiology</topic><topic>Microscopy</topic><topic>Nesterenkonia</topic><topic>New species</topic><topic>nucleic acid hybridization</topic><topic>nucleotide sequences</topic><topic>Nucleotides</topic><topic>Original Paper</topic><topic>Peptidoglycans</topic><topic>Phosphatidylethanolamine</topic><topic>phosphatidylethanolamines</topic><topic>Phospholipids</topic><topic>Phospholipids - analysis</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Physiology</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Seawater</topic><topic>Sequence analysis</topic><topic>Sequence Analysis, DNA</topic><topic>Sodium chloride</topic><topic>species</topic><topic>Taxonomy</topic><topic>xylose</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Aolin</creatorcontrib><creatorcontrib>Cai, Hanqin</creatorcontrib><creatorcontrib>Huang, Ying</creatorcontrib><creatorcontrib>Yang, Quan</creatorcontrib><creatorcontrib>Zhu, Zhenxin</creatorcontrib><creatorcontrib>Zhou, Yan</creatorcontrib><creatorcontrib>Jiang, Mingguo</creatorcontrib><creatorcontrib>Jiang, Yi</creatorcontrib><creatorcontrib>Huang, Wenshan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><jtitle>Archives of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Aolin</au><au>Cai, Hanqin</au><au>Huang, Ying</au><au>Yang, Quan</au><au>Zhu, Zhenxin</au><au>Zhou, Yan</au><au>Jiang, Mingguo</au><au>Jiang, Yi</au><au>Huang, Wenshan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nesterenkonia marinintestina sp. nov., isolated from the fish intestine</atitle><jtitle>Archives of microbiology</jtitle><stitle>Arch Microbiol</stitle><addtitle>Arch Microbiol</addtitle><date>2024-03-01</date><risdate>2024</risdate><volume>206</volume><issue>3</issue><spage>110</spage><epage>110</epage><pages>110-110</pages><artnum>110</artnum><issn>0302-8933</issn><eissn>1432-072X</eissn><abstract>A Gram-positive, aerobic, non-motile, irregular short rod, nonspore-forming actinobacterial strain, designated GX14115
T
, was isolated from fish intestine in Beihai City, Guangxi, China and subjected to a taxonomic polyphasic investigation. Colonies were yellow‒green, circular, smooth, central bulge, convex, opaque and 2.0–3.0 mm in diameter after growth on 2216E medium at 30 °C for 72 h. Growth occurred at 4–45 °C (optimum 30 °C), at pH 4.5–10.0 (optimum pH 7.5) and in the presence of 0–12% NaCl (w/v) (optimum 3.5%). Chemotaxonomic analysis showed that the main menaquinone of strain GX14115
T
was MK-7. The major cellular fatty acids were
anteiso
-C
15:0
(44.8%),
anteiso
-C
17:0
(20.5%), and
iso
-C
15:0
(16%). The whole-cell sugars were galactose and xylose. The peptidoglycan type was
l
-Lys-Gly-
d
-Asp, and the polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), one unknown phospholipid (UP), and one unknown glycolipid (UG). The DNA G + C content of the type of strain was 69.5 mol%. The 16S rRNA gene sequence analysis revealed that strain GX14115
T
is affiliated with the genus
Nesterenkonia
and is closely related to
Nesterenkonia sandarakina
YIM 70009
T
(96.5%) and
Nesterenkonia lutea
YIM 70081
T
(96.8%). The calculated results indicated that the average nucleotide identity (ANI) values of GX14115
T
were 74.49–74.78%, to the two aforementioned type strains, and the digital DNA–DNA hybridization (dDDH) values were 20.1–20.7%. Strain GX14115
T
was proposed as a novel species of the genus
Nesterenkonia
by the physiological, chemotaxonomic, and phylogenetic data, for whose the name is
Nesterenkonia marinintestina
sp. nov. The type of strain is GX14115
T
(= MCCC 1K06658
T
= KCTC 49495
T
).</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>38369673</pmid><doi>10.1007/s00203-023-03825-0</doi><tpages>1</tpages></addata></record> |
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source | MEDLINE; SpringerLink Journals - AutoHoldings |
subjects | Antibiotics Archives & records Bacterial Typing Techniques Biochemistry Biomedical and Life Sciences Biotechnology Cardiolipin Cell Biology chemotaxonomy China Deoxyribonucleic acid DNA DNA, Bacterial - genetics Ecology Fatty Acids - analysis Fish Galactose Genomes genus glycolipids Hybridization Intestine intestines Life Sciences Lipids Menaquinones Microbial Ecology Microbiology Microscopy Nesterenkonia New species nucleic acid hybridization nucleotide sequences Nucleotides Original Paper Peptidoglycans Phosphatidylethanolamine phosphatidylethanolamines Phospholipids Phospholipids - analysis Phylogenetics Phylogeny Physiology RNA, Ribosomal, 16S - genetics rRNA 16S Seawater Sequence analysis Sequence Analysis, DNA Sodium chloride species Taxonomy xylose |
title | Nesterenkonia marinintestina sp. nov., isolated from the fish intestine |
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