Nesterenkonia marinintestina sp. nov., isolated from the fish intestine

A Gram-positive, aerobic, non-motile, irregular short rod, nonspore-forming actinobacterial strain, designated GX14115 T , was isolated from fish intestine in Beihai City, Guangxi, China and subjected to a taxonomic polyphasic investigation. Colonies were yellow‒green, circular, smooth, central bulge, convex, opaque and 2.0–3.0 mm in diameter after growth on 2216E medium at 30 °C for 72 h. Growth occurred at 4–45 °C (optimum 30 °C), at pH 4.5–10.0 (optimum pH 7.5) and in the presence of 0–12% NaCl (w/v) (optimum 3.5%). Chemotaxonomic analysis showed that the main menaquinone of strain GX14115 T was MK-7. The major cellular fatty acids were anteiso -C 15:0 (44.8%), anteiso -C 17:0 (20.5%), and iso -C 15:0 (16%). The whole-cell sugars were galactose and xylose. The peptidoglycan type was l -Lys-Gly- d -Asp, and the polar lipids were phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), one unknown phospholipid (UP), and one unknown glycolipid (UG). The DNA G + C content of the type of strain was 69.5 mol%. The 16S rRNA gene sequence analysis revealed that strain GX14115 T is affiliated with the genus Nesterenkonia and is closely related to Nesterenkonia sandarakina YIM 70009 T (96.5%) and Nesterenkonia lutea YIM 70081 T (96.8%). The calculated results indicated that the average nucleotide identity (ANI) values of GX14115 T were 74.49–74.78%, to the two aforementioned type strains, and the digital DNA–DNA hybridization (dDDH) values were 20.1–20.7%. Strain GX14115 T was proposed as a novel species of the genus Nesterenkonia by the physiological, chemotaxonomic, and phylogenetic data, for whose the name is Nesterenkonia marinintestina sp. nov. The type of strain is GX14115 T (= MCCC 1K06658 T = KCTC 49495 T ).