Changes in the blood redox status of horses subjected to combat training

Combat training of police horses, involving physical activity in the presence of environmental stressors, poses a risk of oxidative stress. This study compared the oxidative imbalance after combat training in horses in the regular police service and in horses that had just been schooled. Blood colle...

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Veröffentlicht in:Research in veterinary science 2024-05, Vol.171, p.105219-105219, Article 105219
Hauptverfasser: Bujok, Jolanta, Pavľak, Alexander, Walski, Tomasz, Zigo, František, Trochanowska-Pauk, Natalia, Bohara, Raghvendra, Miśta, Dorota
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Sprache:eng
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Zusammenfassung:Combat training of police horses, involving physical activity in the presence of environmental stressors, poses a risk of oxidative stress. This study compared the oxidative imbalance after combat training in horses in the regular police service and in horses that had just been schooled. Blood collection was performed immediately after training and after 16 h rest. The activity of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and total antioxidant status (TAS) were determined as the markers of enzymatic antioxidant defence. At the same time, lipid peroxidation (TBARS) and protein carbonylation (Carb) were assessed as oxidation biomarkers. Additionally, oxidative imbalance indexes such as SOD/CAT, SOD/GPx, TBARS/TAS and TBARS/GPx were calculated. Animals during schooling had significantly lower SOD activity in erythrocytes than those experienced. CAT activity in erythrocytes was insignificantly higher immediately after training than during recovery. The SOD/GPx ratio was higher in experienced animals, which may reflect the intra-erythrocyte imbalance between enzymes producing and degrading hydrogen peroxide towards the first one. The concentration of carbonyl groups was significantly higher after the combat training compared to the recovery period in all horses. In inexperienced animals slight increase in TBARS/TAS and TBARS/GPx indexes were observed during the recovery time after exercises, contrary to experienced horses, in which these markers decreased slightly. These results suggest that the oxidative imbalance in inexperienced horses, although less pronounced just after combat training, was more prolonged as compared to horses in regular service. •Imbalance in redox status was observed in horses subjected to mental stressors during moderate physical activity.•Police horses post-combat training show higher SOD antioxidant activity and SOD/GPx index compared to schooling.•Combat training raised carbonyl group levels and catalase activity in all horses, regardless of experience.•Oxidative imbalance after training may be more prolonged in inexperienced horses as compared to horses in regular service.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2024.105219