Rapid on-site detection of Leuconostoc citreum in commercially processed products using loop-mediated isothermal amplification(LAMP) technique

Leuconostoc citreum is widely utilized across numerous industries due to its beneficial properties. Thus, a detection method for this target species is essential to track the presence of L. citreum in commercial products. We developed a rapid, on-site L. citreum detection method using the loop-mediated isothermal amplification (LAMP) technique in this study. The designed LAMP primer set exhibited high specificity, only targeting the intended species among the 76 tested. Moreover, this assay demonstrated a sensitivity of 2 pg of target DNA. To evaluate the LAMP assay's applicability, we selected 20 varied types of processed products. The results confirmed these products' successful and precise detection of L. citreum within 35 min. Additionally, we used samples spiked with L. citreum to determine the detection limit, revealing L. citreum detection up to 102 or 103 CFU/mL. Thus, the LAMP assay developed herein is a specific, sensitive, precise, and rapid on-site detection method for L. citreum in various processed products. •We developed a rapid on-site detection method for Leuconostoc citreum.•Our novel assay exhibits high sensitivity, detecting as little as 2 pg of target DNA.•We detected L. citreum (up to 102 or 103 CFU/mL) in 20 products within 35 min.