Instrument-free and rapid Vibrio parahaemolyticus biosensing based on coupled dual-signal of paper-needle and LnMOF

At present, most reported studies on paper-based sensing devices for food-borne pathogens usually rely on a single signal as the basis for judging the results, accompanied with inaccurate detection results. In this study, we used the lanthanide MOFs being covalently modified with aptamers (Apt-LnMOF...

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Veröffentlicht in:Food control 2024-05, Vol.159, p.110295, Article 110295
Hauptverfasser: Wang, Kuiyu, Sun, Tianying, Gong, Liangke, Zeng, Lanxiang, Liang, Jianwei, Huang, Yayue, Wang, Yuanye, Yang, Tao, Zeng, Hui
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Sprache:eng
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Zusammenfassung:At present, most reported studies on paper-based sensing devices for food-borne pathogens usually rely on a single signal as the basis for judging the results, accompanied with inaccurate detection results. In this study, we used the lanthanide MOFs being covalently modified with aptamers (Apt-LnMOFs) as the sensing and recognition element of Vibrio parahaemolyticus (VP), and the paper-based needle shape device (paper-needle) as the visual signal output carrier. Finally, an accurate detection strategy for VP was successfully constructed. In terms of signal output, the two signal styles of Apt-LnMOFs diffusion distance on the paper-needle and R value (measured by the RGB analysis software of smartphone) are coupled to jointly improve the accuracy of detection results. The method is simple and fast, and the detection accuracy in real samples is 91%. This strategy is very suitable for on-site rapid detection of pathogens, and does not require large professional instruments. [Display omitted] •This method does not require the aid of instruments and professional operation.•The samples can be automatically diffused on the paper-needle device.•The coupled dual-signal jointly improved the accuracy of detection results.•Hand-held paper-needle pathogen analysis device is easy to use and inexpensive.
ISSN:0956-7135
1873-7129
DOI:10.1016/j.foodcont.2024.110295