Knema retusa is antibacterial and antibiofilm against antibiotic resistant Staphylococcus aureus and S. haemolyticus isolated in bovine mastitis

This study aimed to assess antibacterial activity of Knema retusa wood extract (KRe) against antibiotic resistant staphylococci which are causative agents of bovine mastitis. From 75 cases of intramammary infections in dairy cows, 66 staphylococcal isolates were collected, including 11 Staphylococcu...

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Veröffentlicht in:	Veterinary research communications 2023-06, Vol.47 (2), p.523-538
Hauptverfasser:	Chuprom, Julalak, Kidsin, Kamchai, Sangkanu, Suthinee, Nissapatorn, Veeranoot, Wiart, Christophe, de Lourdes Pereira, Maria, Wongtawan, Tuempong, Daus, Mareena, Sotthibandhu, Dennapa Saeloh, Tipmanee, Varomyalin, Paul, Alok K., Scholfield, Charles Norman, Zulkipli, Masyitah Binti, Abdullah, Nor Hayati, Mitsuwan, Watcharapong
Format:	Artikel
Sprache:	eng
Schlagworte:	adhesion Animals Anti-Bacterial Agents - pharmacology Antibacterial activity antibacterial properties Antibiotic resistance Antibiotics bacterial adhesion biofilm Biofilms Biomedical and Life Sciences bovine mastitis Cattle Cattle Diseases Coagulase Female Free energy Life Sciences Mastitis Mastitis, Bovine - drug therapy Mastitis, Bovine - microbiology Methicillin-Resistant Staphylococcus aureus Microbial Sensitivity Tests - veterinary Minimum inhibitory concentration Molecular Docking Simulation species Staphylococcal Infections - drug therapy Staphylococcal Infections - microbiology Staphylococcal Infections - veterinary Staphylococcus Staphylococcus aureus Veterinary Medicine/Veterinary Science wood extractives Zoology
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Zusammenfassung:	This study aimed to assess antibacterial activity of Knema retusa wood extract (KRe) against antibiotic resistant staphylococci which are causative agents of bovine mastitis. From 75 cases of intramammary infections in dairy cows, 66 staphylococcal isolates were collected, including 11 Staphylococcus aureus isolates (17%) and 55 coagulase-negative staphylococci (83%). Sixty isolates (91%) formed strong biofilms. KRe had minimal inhibitory concentrations (MIC) and minimal bactericidal concentrations (MBC) against the isolates ranging 32–256 ug/mL and 64–512 ug/mL, respectively. Two-hour KRe exposures at 4×MIC, viabilities of S. aureus and S. haemolyticus decreased by 3 log 10 compared to the control. Scanning EM (SEM) showed that KRe disrupted the bacterial cells of both species. KRe at 1/16×MIC significantly inhibited biofilm formation (P
ISSN:	0165-7380 1573-7446
DOI:	10.1007/s11259-022-09999-0