Decolorization and detoxication of malachite green by engineered Saccharomyces cerevisiae expressing novel thermostable laccase from Trametes trogii

[Display omitted] •A novel thermostable laccase Lcc1 was expressed in Saccharomyces cerevisiae for MG decolorization and detoxification.•Lcc1 exhibited strong thermal stability and showed a high tolerance to organic solvents.•The decolorization rate of the engineered strain RCL to MG reached 95.10% and can decolorize different types of dyes.•Cytotoxicity analysis and metabolism analysis confirmed non-toxic by-products of MG biodegradation.•The engineered strain RCL has a great degrading effect on industrial wastewater. Malachite Green (MG) is a widely used industrial dye that is hazardous to health. Herein, the decolourisation and detoxification of MG were achieved using the engineered Saccharomyces cerevisiae expressing novel thermostable laccase lcc1 from Trametes trogii. The engineered strain RCL produced a high laccase activity of 121.83 U L-1. Lcc1 was stable at temperatures ranging from 20 ℃ to 60 ℃ and showed a high tolerance to organic solvents. Moreover, Lcc1 could decolorize different kinds of dyes (azo, anthraquinone and triphenylmethane), among which, the decolorization ability of MG is the highest, reaching 95.10 %, and the decolorization rate of other triphenylmethane dyes also over 50 %. The RCL decolorized about 95 % of 50 mg L-1 of MG dye in 10 h at 30 ℃. The MG degradation products were analyzed. The industrial application potential of the RCL was evaluated by treating industrial wastewater and the decolourisation rates were over 90 %.