Proanthocyanidins and Phenolic Compounds from the Twigs of Salix chaenomeloides and Their Anti-Lipogenic Effects on 3T3-L1 Preadipocytes

The present study investigated potential bioactive natural products from the EtOH extract of twigs using column chromatography, leading to the isolation of six compounds ( - ), which were characterized as two proanthocyanidins, procyanidin B ( ) and procyanidin B ( ), and four phenolic compounds, 4-hydroxybenzoic acid -D-glucosyl ester ( ), di- -methylcrenatin ( ), -coumaric acid glucoside ( ), and syringin ( ) by the comparison of their NMR spectra with the reported data and high-resolution (HR)-electrospray ionization mass spectroscopy (ESI-MS) analysis. We investigated the potential of six compounds ( - ) to inhibit adipogenesis in 3T3-L1 preadipocytes, which showed that the compounds ( - ) significantly reduced lipid accumulation in 3T3-L1 adipocytes without affecting cell proliferation. Notably, compound demonstrated a remarkable 60% and 90% reduction in lipid levels with 50 and 100 µM treatments, respectively. Oil Red O staining results indicated that compound significantly inhibits the formation of lipid droplets, comparable to the effect of T863, an inhibitor of triglyceride used as a positive control, in adipocytes. Compound had no effect on the regulators PPARγ, C/EBPα, and SREBF1 of adipocyte differentiation in 3T3-L1 preadipocytes, but compound activated the fatty acid oxidation regulator, PPARα, compared to the lipogenic-induced control. It also suppressed fatty acid synthesis by downregulating the expression of fatty acid synthase (FAS). Finally, compound induced the mRNA and protein levels of CPT1A, an initial marker of mitochondrial fatty acid oxidation in 3T3-L1. This finding substantiates the anti-lipogenic and lipolytic effects of procyanidin B ( ) in 3T3-L1 preadipocytes, emphasizing its pivotal role in modulating obesity-related markers.