Microbial crosstalk with dermal immune system: A review on emerging analytical methods for macromolecular detection and therapeutics

According to global health metrics, clinical symptoms such as cellulitis and pyoderma associated with skin diseases are a significant burden worldwide, affecting 2.2 million disability-adjusted life years in 2020. There is a strong correlation between the commensal bacteria and the host immune system. Classical methods deployed in dermal biofilm crosstalk studies often hamper many individuals from early diagnosis and rationalized therapy. Herein, the present report aims to study the role of skin microbiota and mechanisms of microbial crosstalk with host immune system. The emerging analytical tools devised for sensor/biosensor platforms, including molecularly imprinted polymers, microarrays, aptamers, CRISPR-cas9, and optical/electrochemical approaches, are discussed as alternative methods for important biomarker analysis. Further, the types and characteristics of microorganism-derived macromolecules and the recent skin organoid toward personalized therapy are highlighted. This information will largely benefit researchers involved in the pathophysiology of skin disease, wound dressing materials, including diagnostic and healing patch designs, in addition to biological macromolecules devoted to wound repair. [Display omitted]