Diagnostic value of SAT-TB in stool and urine samples for intestinal and urinary tuberculosis

•Sensitivity and specificity for stool samples: 53.33 %, 93.88 % (SAT-TB), 60 %, 81.63 % (Xpert).•SAT-TB and Xpert showed moderate consistency with clinical diagnosis (Kappa: 0.48, 0.42).•SAT-TB sensitivity, specificity, and AUC for urine samples: 27.27 %, 98.28 %, 0.628.•Combining SAT-TB, Xpert, and MGIT960 culture improved diagnostic efficacy for stool samples (AUC: 0.823).•SAT-TB had the lowest diagnostic efficacy for urine samples; combined methods increased AUC. The simultaneous amplification/testing for tuberculosis (SAT-TB) targets specific 16s rRNA for detecting Mycobacterium tuberculosis in real-time. To evaluate SAT-TB's performance in detecting intestinal and urinary TB using stool and urine samples. Stool (94) and urine samples (69) (From 2021 to 2022), were collected from pulmonary combined with suspected intestinal or urinary tuberculosis. Simultaneous detection of Mycobacterium tuberculosis was performed using the SAT-TB method, Xpert MTB/RIF assay, and MGIT960 culture. For stool samples, the sensitivity, specificity, and area under the curve (AUC) were 53.33 %, 93.88 %, and 0.736 for SAT-TB; 60 %, 81.63 %, and 0.708 for Xpert; and 40 %, 95.92 %, and 0.680 for MGIT960. For urine samples, the sensitivity, specificity, and AUC for SAT-TB, Xpert, and MGIT960 were 27.27 %, 98.28 %, 0.628; 54.55 %, 96.35 %, 0.755; and 45.45 %, 100 %, 0.727, respectively. ROC analysis indicated that SAT-TB had the highest diagnostic efficacy for stool samples when tested individually.