A fluorescent probe based on pyrazoline with significant Stokes shifts for the detection of Cu2+ ions and its applications

A fluorescent probe based on pyrazoline with significant Stokes shifts for the detection of Cu2+ ions and its applications

A simple pyrazoline derivative BBD has been synthesized for detecting Cu2+ in EtOH/HEPES (v/v = 1 : 1, pH = 7). The probe has high selectivity for Cu2+ by quenching the fluorescence intensity and was sensitive to pH. When excited at 398 nm, fluorescence is emitted at 520 nm with a Stokes shift of 12...

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Hauptverfasser: Shang, Yajing, Wu, Xinghu, Luo, Haoting
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description A simple pyrazoline derivative BBD has been synthesized for detecting Cu2+ in EtOH/HEPES (v/v = 1 : 1, pH = 7). The probe has high selectivity for Cu2+ by quenching the fluorescence intensity and was sensitive to pH. When excited at 398 nm, fluorescence is emitted at 520 nm with a Stokes shift of 122 nm, which is larger than that of other reported pyrazoline skeleton probes. The detection limit (LOD) of the probe for Cu2+ is 0.862 μM which is lower than the value of the WHO limit (31.5 μM) in water. The 2 : 1 binding mode and probable recognition mechanism of the probe with Cu2+ were confirmed by Job's plot, IR, ESI-MS, and TD-DFT analysis. Moreover, the probe has been successfully applied to actual water samples as well as cell and zebrafish imaging.A simple pyrazoline derivative BBD has been synthesized for detecting Cu2+ in EtOH/HEPES (v/v = 1 : 1, pH = 7). The probe has high selectivity for Cu2+ by quenching the fluorescence intensity and was sensitive to pH. When excited at 398 nm, fluorescence is emitted at 520 nm with a Stokes shift of 122 nm, which is larger than that of other reported pyrazoline skeleton probes. The detection limit (LOD) of the probe for Cu2+ is 0.862 μM which is lower than the value of the WHO limit (31.5 μM) in water. The 2 : 1 binding mode and probable recognition mechanism of the probe with Cu2+ were confirmed by Job's plot, IR, ESI-MS, and TD-DFT analysis. Moreover, the probe has been successfully applied to actual water samples as well as cell and zebrafish imaging.
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The probe has high selectivity for Cu2+ by quenching the fluorescence intensity and was sensitive to pH. When excited at 398 nm, fluorescence is emitted at 520 nm with a Stokes shift of 122 nm, which is larger than that of other reported pyrazoline skeleton probes. The detection limit (LOD) of the probe for Cu2+ is 0.862 μM which is lower than the value of the WHO limit (31.5 μM) in water. The 2 : 1 binding mode and probable recognition mechanism of the probe with Cu2+ were confirmed by Job's plot, IR, ESI-MS, and TD-DFT analysis. Moreover, the probe has been successfully applied to actual water samples as well as cell and zebrafish imaging.A simple pyrazoline derivative BBD has been synthesized for detecting Cu2+ in EtOH/HEPES (v/v = 1 : 1, pH = 7). The probe has high selectivity for Cu2+ by quenching the fluorescence intensity and was sensitive to pH. When excited at 398 nm, fluorescence is emitted at 520 nm with a Stokes shift of 122 nm, which is larger than that of other reported pyrazoline skeleton probes. The detection limit (LOD) of the probe for Cu2+ is 0.862 μM which is lower than the value of the WHO limit (31.5 μM) in water. The 2 : 1 binding mode and probable recognition mechanism of the probe with Cu2+ were confirmed by Job's plot, IR, ESI-MS, and TD-DFT analysis. 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The probe has high selectivity for Cu2+ by quenching the fluorescence intensity and was sensitive to pH. When excited at 398 nm, fluorescence is emitted at 520 nm with a Stokes shift of 122 nm, which is larger than that of other reported pyrazoline skeleton probes. The detection limit (LOD) of the probe for Cu2+ is 0.862 μM which is lower than the value of the WHO limit (31.5 μM) in water. The 2 : 1 binding mode and probable recognition mechanism of the probe with Cu2+ were confirmed by Job's plot, IR, ESI-MS, and TD-DFT analysis. Moreover, the probe has been successfully applied to actual water samples as well as cell and zebrafish imaging.A simple pyrazoline derivative BBD has been synthesized for detecting Cu2+ in EtOH/HEPES (v/v = 1 : 1, pH = 7). The probe has high selectivity for Cu2+ by quenching the fluorescence intensity and was sensitive to pH. When excited at 398 nm, fluorescence is emitted at 520 nm with a Stokes shift of 122 nm, which is larger than that of other reported pyrazoline skeleton probes. The detection limit (LOD) of the probe for Cu2+ is 0.862 μM which is lower than the value of the WHO limit (31.5 μM) in water. The 2 : 1 binding mode and probable recognition mechanism of the probe with Cu2+ were confirmed by Job's plot, IR, ESI-MS, and TD-DFT analysis. 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When excited at 398 nm, fluorescence is emitted at 520 nm with a Stokes shift of 122 nm, which is larger than that of other reported pyrazoline skeleton probes. The detection limit (LOD) of the probe for Cu2+ is 0.862 μM which is lower than the value of the WHO limit (31.5 μM) in water. The 2 : 1 binding mode and probable recognition mechanism of the probe with Cu2+ were confirmed by Job's plot, IR, ESI-MS, and TD-DFT analysis. Moreover, the probe has been successfully applied to actual water samples as well as cell and zebrafish imaging.</abstract><doi>10.1039/d4ay01772b</doi></addata></record>
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title A fluorescent probe based on pyrazoline with significant Stokes shifts for the detection of Cu2+ ions and its applications
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