Resident synovial macrophages in synovial fluid: Implications for immunoregulation

Resident synovial macrophages (RSMs) are anti-inflammatory, self-renewing macrophages that provide physical immune sequestration of the joint space from the peripheral immune system. Increased permeability of this structure is associated with peripheral immune cells in the synovial fluid (SF). Direct measures of synovial barrier integrity are possible with tissue histology, but after barrier breakdown, if these cells perpetuate or initiate chronic inflammation in SF remains unknown. We sought to identify RSM in human SF as an indirect measure of synovial barrier integrity. To validate findings, we created a novel ex vivo explant model using human synovium. scRNA-seq revealed these SF RSMs upregulated pro-fibrotic and pro-osteoclastic pathways in inflammatory arthritis, but not septic arthritis. Increased frequencies of RSMs in SF was associated with increased sRANKL regardless of underlying pathology. These findings suggest the frequency of RSMs in SF may correlate with synovial barrier damage and in turn, potential damage to joint structures. [Display omitted] •Resident Synovial Macrophages (RSMs) leave the synovium and enter the synovial fluid (SF).•we present a novel transwell system to simulate the joint space ex vivo.•Increasing RSM in the SF corresponds with increasing sRANKL in SF.•RSM-like cells in inflammatory SF produce pro-fibrotic and pro-osteoclastic transcripts.