Innovative transgenic zebrafish biosensor for heavy metal detection

Heavy metal contamination is an urgent environmental issue that poses a significant threat to human health and the ecosystem. To mitigate the adverse impacts of heavy metal pollution, the aim of this research was to develop genetically engineered zebrafish as biosensors, which offer a promising alternative for detecting heavy metal exposure, specifically Cd2⁺ and Zn2⁺. A novel heavy metal-sensitive gene construct metallothionine 2 promoter with DsRed reporter gene (mt2-DsRed2) was synthesized and integrated into zebrafish embryos using a Tol2 transposon transposase system with the transgenic zebrafish line subjected to biosensing applications for Cd2+ and Zn2+. The biosensor showed specific responses with linear correlation heavy metal concentration and DsRed fluorescence signal for Cd2+ and Zn2+ with (p