An Optimized Ex Vivo n-3 PUFA Supplementation Strategy for Primary Human Macrophages Shows That DHA Suppresses Prostaglandin E2 Formation

An Optimized Ex Vivo n-3 PUFA Supplementation Strategy for Primary Human Macrophages Shows That DHA Suppresses Prostaglandin E2 Formation

Evidence suggests beneficial effects of long-chain n-3 polyunsaturated fatty acids (PUFAs) in inflammatory diseases. However, the underlying mechanisms are still subject of research. For this purpose, we developed an ex vivo n-3 PUFA supplementation strategy. M2-like macrophages were supplemented fo...

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Veröffentlicht in:Molecular nutrition & food research 2024-12, p.e202400716
Hauptverfasser: Kirchhoff, Rebecca, Kampschulte, Nadja, Rothweiler, Carina, Rohwer, Nadine, Weylandt, Karsten-Henrich, Schebb, Nils Helge
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Sprache:eng
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Zusammenfassung:Evidence suggests beneficial effects of long-chain n-3 polyunsaturated fatty acids (PUFAs) in inflammatory diseases. However, the underlying mechanisms are still subject of research. For this purpose, we developed an ex vivo n-3 PUFA supplementation strategy. M2-like macrophages were supplemented for 2-3 days with 20-40 µM docosahexaenoic acid (DHA) during differentiation. Quality parameters include
ISSN:1613-4125
1613-4133
1613-4133
DOI:10.1002/mnfr.202400716