Lectin from microalgae: Non-toxic to human cells and effective against Leishmania

New leishmanicidal products are needed for the treatment to be effective, since current drugs are toxic to healthy human/animal cells and have low efficacy against the parasite. Bioactive compounds from microalgae, such as lectins, can be explored as new anti-Leishmania candidates. This study aimed to evaluate the cytotoxic and anti-Leishmania action of the cell extract (CE) and lectin (CVU) from Chlorella vulgaris biomass. CVU purified from CE was stable within alkaline pH and temperature below 50 °C. CVU showed specificity to the carbohydrate D-Galactose, which is found in the membrane of Leishmania. CE (IC50 = 161.4 μg/mL) and CVU (IC50 = 66.32 μg/mL) exhibited effects against the promastigotes of L. braziliensis, and scanning electron microscopy confirmed alterations the shape and size in treated parasites. CE showed a higher selectivity index than CVU on L. braziliensis promastigotes. These results demonstrate the potential of C. vulgaris as a source of active molecules that can be investigated as therapeutic candidates. •Cell extract (CE) and lectin (CVU) from Chlorella vulgaris were able to inhibit the growth of Leishmania braziliensis•Both CE and CVU change the body shape of Leishmania and non-toxic to host cells•CE and CVU may be used as a source of biomolecules to leishmaniasis