Widespread distribution of chlorophyll f-producing Leptodesmis cyanobacteria

Chlorophyll (Chl) f was reported as the fifth Chl in oxygenic photoautotrophs. Chlorophyll f production expanded the utilization of photosynthetically active radiation into the far-red light (FR) region in some cyanobacterial genera. In this study, 11 filamentous cyanobacterial strains were isolated from FR-enriched habitats, including hydrophyte, moss, shady stone, shallow ditch, and microbial mat across Central and Southern China. Polyphasic analysis classified them into the same genus of Leptodesmis and further recognized them as four new species, including Leptodesmis atroviridis sp. nov., Leptodesmis fuscus sp. nov., Leptodesmis olivacea sp. nov., and Leptodesmis undulata sp. nov. These cyanobacteria had absorption peaks beyond 700 nm due to Chl f production and red-shifted phycobiliprotein complexes under FR conditions. All but L. undulata produced phycoerythrin and showed varying degrees of a reddish-brown to dark green color under white light conditions. However, the phycoerythrin contents were sharply decreased under FR conditions, and these three Leptodesmis species appeared green. In summary, the Leptodesmis genus contains diverse species with the capacity to synthesize Chl f and is likely a ubiquitous group of Chl f-producing cyanobacteria.