Microbial adaptation and genetic modifications for enhanced remediation in low-permeability soils

Low-permeability soils, characterized by fine texture and high clay content, pose significant challenges to traditional soil remediation techniques due to limited hydraulic conductivity, restricted nutrient flow, and reduced oxygen availability. These unique properties enable low-permeability soils to function as natural barriers in environmental protection; however, they also trap contaminants, making traditional remediation efforts challenging. This review synthesizes current knowledge on microbial adaptation and genetic engineering approaches that enhance the effectiveness of bioremediation in such environments. Key microbial adaptations, including anaerobic metabolism, extracellular enzyme production, and stress response mechanisms, allow individual microbes to adapt in low-permeability soils. Additionally, community-level strategies like microhabitat creation, biofilm formation, and functional redundancy further support microbial resilience. Advancements in genetic engineering now enable the modification of microbial traits—such as soil adhesion, nutrient utilization, and stress tolerance—to enhance bioremediation efficacy. Synthetic biology techniques further allow for the design of tailored microbial consortia that work cooperatively to degrade contaminants in complex soil matrices. This review highlights the integration of microbial and genetic engineering strategies, offering a comprehensive overview that informs current practices and guides future research in low-permeability soil remediation. [Display omitted] •Remediation of contaminated low-permeability soils is exceptionally challenging.•Individuals and microbial communities thrive differently in low-permeability soils.•Genetic tools enhance microbial resilience in low-permeability soil remediation.•Tailored microbial consortia boost resilience in low-permeability soils.•Comprehensive insights into overcoming challenges in soil remediation.