Inoculation of thermophilic bacteria from giant panda feces into cattle manure reduces gas emissions and decreases resistance gene prevalence in short-term composting

Here, thermophilic bacteria (TB) with cellulose degradation functions were screened from composting panda feces and applied to cattle manure composting. TB (Aeribacillus pallidus G5 and Parageobacillus toebii G12) inoculation led to remarkable improvement of the compost temperature, prolonging of the thermophilic stage and shortening of the composting process, resulting in increased manure harmlessness (GI ≥ 70%), compost humification, and greenhouse gas emission reduction (14.19%–22.57%), compared with the control compost, within 15 days of composting. In particular, G5 inoculation reduced NH3 emissions by 41.97% relative to control composts over 15 days. G5 was capable of rapidly colonizing in the composts, and its inoculation immediately enriched the genera of Firmicutes, and simultaneously decreased the genera of Proteobacteria, contributing to the elimination of harmful microorganisms. Notably, this strain lacked antibiotic resistance genes, and the absolute abundances of resistance genes and mobile genetic genes (MGEs) decreased the most (by 80.84%). Metagenomic analysis revealed that enzymes capable of producing CO2, N2O, and NH3 were generally inhibited, while CO2 fixation and N2O and NH3 reduction enzymes were enriched in the G5 compost, since metagenome-assembled genomes of Proteobacteria harbored more key genes and enzymes in complete pathways for producing N2O, NH3, and CO2. Moreover, Proteobacteria, such as Pseudomonas and Halopseudomonas, were the main host of resistance genes and MGEs. Overall, the gas emission could be reduced, and more efficient control of resistance genes could be achieved by inhibited the abundance of Proteobacteria during composting. This study provides a safe and effective microbial agent (A. pallidus) for manure treatment. [Display omitted] •Inoculation of TB accelerated the maturity and harmlessness of compost over 15 days.•A. pallidus reducd GHG and NH3 emissions by 22.57% and 41.97%, respectively.•A. pallidus decreased the abundances of resistance genes and MGEs.•A. pallidus enriched the abundance of Firmicutes while inhibiting the abundance of Proteobacteria.•Proteobacteria are responsible for gas production and supercarriers of ARGs, HMRGs, MGEs, and VFGs.