Glutathione mitigates aluminum toxicity in root-apex transition zone of rice through reducing aluminum absorption and maintaining redox balance

Aluminium (Al) toxicity is recognized as a major constraint on crop growth and production in acidic soils, and the transition zone (TZ) of plant root apex emerges as the major perception site of Al toxicity. Glutathione (GSH) is reported to be involved in plant responses to various abiotic stresses, but its role and mechanism under Al stress remain unknown. Here, we found that GSH significantly mitigated Al toxicity on rice as revealed by the promotion of root elongation, reduction of oxidative stress and Al absorption. GSH application scavenged Al-induced H2O2 burst by activating the ascorbate (AsA)-GSH cycle and proline synthesis in root-apex TZ, thereby alleviating oxidative stress. GSH effectively reduced Al-induced pectin increment and inhibits the H2O2-induced pectin methylesterase (PME) activity and demethylesterification degree in root-apex TZ, leading to a reduction in Al binding sites and subsequently Al deposition in cell walls, thereby attenuating the inhibitory effect of Al toxicity on cell elongation. In addition, GSH-derived phytochelatins (PCs) promoted the vacuolar Al sequestration in root-apex TZ, which alleviated Al toxicity to the cytoplasm. Taken together, our results indicate a mechanism underlying how GSH alleviates Al toxicity through influencing redox state and Al absorption in rice root-apex TZ. [Display omitted] •GSH can improve root elongation and development under Al stress.•GSH relieves oxidative stress in root-apex TZ by activating AsA-GSH cycle and proline biosynthesis.•GSH decreases Al deposition in root-apex TZ cell wall by reducing pectin content and pectin demethylesterification.•GSH-derived phytochelatins (PCs) promote the vacuolar Al sequestration in root-apex TZ.