Comprehensive analysis of the PbrTBL gene family and functional analysis of PbrTBL43 under Botryosphaeria dothidea infection in Pyrus bretschneideri

The TBL (Trichome Birefringence-Like) gene family, which participates in the initiation of trichomes and the acetylation of xylan in a variety of plant species, plays a significant role in plant biology. However, there is little information regarding TBL family members in pear (Pyrus bretschneideri Rehd). Here, 65 PbrTBL genes were identified in Pyrus bretschneideri genome. Phylogenetic, gene structure, expression pattern and cis-element of promoter analysis were performed and compared. Expression profiling across different tissues and in response to Botryosphaeria dothidea (B. dothidea) infection highlighted the dynamic and coordinated response of PbrTBL genes, with PbrTBL43 showing significant upregulation. Subcellular localization of PbrTBL43 to the plasma membrane and the enhanced susceptibility to B. dothidea infection upon PbrTBL43 silencing further support its role in pathogen resistance. This study enhances our understanding of the PbrTBL gene family's multifaceted involvement in pear biology and provides a foundation for future research aimed at improving pear resistance to diseases and environmental challenges.