New insight in the biotreatment of produced water: Pre-oxidation paves a rapid pathway for substrate selection in microbial community

The deep treatment of produced water (PW) had emerged as a formidable challenge due to the coexistence of hydrocarbons, surfactants, ammonium nitrogen, and other refractory organics. On the basis of the pre-oxidation coupled heterotrophic ammonia assimilation (PHAA) system constructed in previous research, this work refined the catalyst selection and reduced the hydraulic retention time. The stable running PHAA system removed 96.2 % of total organic carbon (TOC). The study simulated the effects of organic loading fluctuations on the system and dissected the mechanism of pre-oxidation process and its contribution to microbial community. Pre-oxidation significantly improved the ability of microbial community to handle loading shocks and improved organic degradation efficiency in PW during long-term reactor operation. The PHAA process effectively removed medium to long chain alkanes above C24 in PW and proposed potential degradation pathways and direction. The determination of hydrocarbon enzymes activity showed that pre-oxidation changed the substrate selection, making more aldehydes available as auxiliary carbon sources for microorganisms. Pre-oxidation also enriched and preserved microbial diversity, facilitating the accumulation of functional microorganisms in the PHAA process. [Display omitted] •Developed a pyrrhotite/PMS pre-oxidation combined microbial system to treat PW.•The PHAA combined process achieved 96.2 % TOC removal in PW.•Pre-oxidation enhanced the activity of ALDH in the microbial community.•Richer microbial substrates promoted the conversion of hydrocarbons.•Pre-oxidation enriched and protected the diversity of microbial community.