Calculating point spread functions: methods, pitfalls, and solutions

The knowledge of the exact structure of the optical system point spread function (PSF) enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF mode...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Optics express 2024-07, Vol.32 (16), p.27278
Hauptverfasser: Holinirina Dina Miora, Ratsimandresy, Rohwer, Erich, Kielhorn, Martin, Sheppard, Colin, Bosman, Gurthwin, Heintzmann, Rainer
Format: Artikel
Sprache:eng
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue 16
container_start_page 27278
container_title Optics express
container_volume 32
creator Holinirina Dina Miora, Ratsimandresy
Rohwer, Erich
Kielhorn, Martin
Sheppard, Colin
Bosman, Gurthwin
Heintzmann, Rainer
description The knowledge of the exact structure of the optical system point spread function (PSF) enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF models fall into a sampling pitfall near the center position, yielding to the violation of energy conservation. In this work, we present a novel, to the best of our knowledge, Fourier-based techniques for computing vector PSF and compare them to the state-of-the-art. Our methods are shown to satisfy the physical condition of the imaging process. They are reproducible, computationally efficient, easy to implement, and easy to modify to represent various imaging modalities.
doi_str_mv 10.1364/OE.523532
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_3128820794</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>3128820794</sourcerecordid><originalsourceid>FETCH-LOGICAL-c210t-8c814cdb434661e79cae3c817ed87274dd02561db0bcf4fbdee66f2b6f9c5373</originalsourceid><addsrcrecordid>eNpNkMlOwzAARC0EolA48AMoR5BI8RYv3FApi1Spl94txwsEOXGInQN_T6AFcZrR6GkOD4ALBBeIMHq7WS0qTCqCD8AJgpKWFAp--K_PwGlK7xAiyiU_BjMiKyIqJk_Aw1IHMwadm-616GPT5SL1g9O28GNnchO7dFe0Lr9Fm26KvslehzA13dkixTD-EGfgaJqTO9_nHGwfV9vlc7nePL0s79elwQjmUhiBqLE1JZQx5Lg02pFp484Kjjm1FuKKIVvD2njqa-scYx7XzEtTEU7m4Gp32w_xY3Qpq7ZJxoWgOxfHpAjCQmDIJZ3Q6x1qhpjS4Lzqh6bVw6dCUH07U5uV2jmb2Mv97Vi3zv6Rv5LIF_jJZt8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>3128820794</pqid></control><display><type>article</type><title>Calculating point spread functions: methods, pitfalls, and solutions</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Holinirina Dina Miora, Ratsimandresy ; Rohwer, Erich ; Kielhorn, Martin ; Sheppard, Colin ; Bosman, Gurthwin ; Heintzmann, Rainer</creator><creatorcontrib>Holinirina Dina Miora, Ratsimandresy ; Rohwer, Erich ; Kielhorn, Martin ; Sheppard, Colin ; Bosman, Gurthwin ; Heintzmann, Rainer</creatorcontrib><description>The knowledge of the exact structure of the optical system point spread function (PSF) enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF models fall into a sampling pitfall near the center position, yielding to the violation of energy conservation. In this work, we present a novel, to the best of our knowledge, Fourier-based techniques for computing vector PSF and compare them to the state-of-the-art. Our methods are shown to satisfy the physical condition of the imaging process. They are reproducible, computationally efficient, easy to implement, and easy to modify to represent various imaging modalities.</description><identifier>ISSN: 1094-4087</identifier><identifier>EISSN: 1094-4087</identifier><identifier>DOI: 10.1364/OE.523532</identifier><identifier>PMID: 39538569</identifier><language>eng</language><publisher>United States</publisher><ispartof>Optics express, 2024-07, Vol.32 (16), p.27278</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c210t-8c814cdb434661e79cae3c817ed87274dd02561db0bcf4fbdee66f2b6f9c5373</cites><orcidid>0000-0002-0402-6561 ; 0000-0003-1108-8346 ; 0000-0002-4950-1936 ; 0000-0002-0792-4607 ; 0000-0001-6102-694X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,864,27915,27916</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39538569$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Holinirina Dina Miora, Ratsimandresy</creatorcontrib><creatorcontrib>Rohwer, Erich</creatorcontrib><creatorcontrib>Kielhorn, Martin</creatorcontrib><creatorcontrib>Sheppard, Colin</creatorcontrib><creatorcontrib>Bosman, Gurthwin</creatorcontrib><creatorcontrib>Heintzmann, Rainer</creatorcontrib><title>Calculating point spread functions: methods, pitfalls, and solutions</title><title>Optics express</title><addtitle>Opt Express</addtitle><description>The knowledge of the exact structure of the optical system point spread function (PSF) enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF models fall into a sampling pitfall near the center position, yielding to the violation of energy conservation. In this work, we present a novel, to the best of our knowledge, Fourier-based techniques for computing vector PSF and compare them to the state-of-the-art. Our methods are shown to satisfy the physical condition of the imaging process. They are reproducible, computationally efficient, easy to implement, and easy to modify to represent various imaging modalities.</description><issn>1094-4087</issn><issn>1094-4087</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpNkMlOwzAARC0EolA48AMoR5BI8RYv3FApi1Spl94txwsEOXGInQN_T6AFcZrR6GkOD4ALBBeIMHq7WS0qTCqCD8AJgpKWFAp--K_PwGlK7xAiyiU_BjMiKyIqJk_Aw1IHMwadm-616GPT5SL1g9O28GNnchO7dFe0Lr9Fm26KvslehzA13dkixTD-EGfgaJqTO9_nHGwfV9vlc7nePL0s79elwQjmUhiBqLE1JZQx5Lg02pFp484Kjjm1FuKKIVvD2njqa-scYx7XzEtTEU7m4Gp32w_xY3Qpq7ZJxoWgOxfHpAjCQmDIJZ3Q6x1qhpjS4Lzqh6bVw6dCUH07U5uV2jmb2Mv97Vi3zv6Rv5LIF_jJZt8</recordid><startdate>20240729</startdate><enddate>20240729</enddate><creator>Holinirina Dina Miora, Ratsimandresy</creator><creator>Rohwer, Erich</creator><creator>Kielhorn, Martin</creator><creator>Sheppard, Colin</creator><creator>Bosman, Gurthwin</creator><creator>Heintzmann, Rainer</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0402-6561</orcidid><orcidid>https://orcid.org/0000-0003-1108-8346</orcidid><orcidid>https://orcid.org/0000-0002-4950-1936</orcidid><orcidid>https://orcid.org/0000-0002-0792-4607</orcidid><orcidid>https://orcid.org/0000-0001-6102-694X</orcidid></search><sort><creationdate>20240729</creationdate><title>Calculating point spread functions: methods, pitfalls, and solutions</title><author>Holinirina Dina Miora, Ratsimandresy ; Rohwer, Erich ; Kielhorn, Martin ; Sheppard, Colin ; Bosman, Gurthwin ; Heintzmann, Rainer</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c210t-8c814cdb434661e79cae3c817ed87274dd02561db0bcf4fbdee66f2b6f9c5373</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Holinirina Dina Miora, Ratsimandresy</creatorcontrib><creatorcontrib>Rohwer, Erich</creatorcontrib><creatorcontrib>Kielhorn, Martin</creatorcontrib><creatorcontrib>Sheppard, Colin</creatorcontrib><creatorcontrib>Bosman, Gurthwin</creatorcontrib><creatorcontrib>Heintzmann, Rainer</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Optics express</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Holinirina Dina Miora, Ratsimandresy</au><au>Rohwer, Erich</au><au>Kielhorn, Martin</au><au>Sheppard, Colin</au><au>Bosman, Gurthwin</au><au>Heintzmann, Rainer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Calculating point spread functions: methods, pitfalls, and solutions</atitle><jtitle>Optics express</jtitle><addtitle>Opt Express</addtitle><date>2024-07-29</date><risdate>2024</risdate><volume>32</volume><issue>16</issue><spage>27278</spage><pages>27278-</pages><issn>1094-4087</issn><eissn>1094-4087</eissn><abstract>The knowledge of the exact structure of the optical system point spread function (PSF) enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF models fall into a sampling pitfall near the center position, yielding to the violation of energy conservation. In this work, we present a novel, to the best of our knowledge, Fourier-based techniques for computing vector PSF and compare them to the state-of-the-art. Our methods are shown to satisfy the physical condition of the imaging process. They are reproducible, computationally efficient, easy to implement, and easy to modify to represent various imaging modalities.</abstract><cop>United States</cop><pmid>39538569</pmid><doi>10.1364/OE.523532</doi><orcidid>https://orcid.org/0000-0002-0402-6561</orcidid><orcidid>https://orcid.org/0000-0003-1108-8346</orcidid><orcidid>https://orcid.org/0000-0002-4950-1936</orcidid><orcidid>https://orcid.org/0000-0002-0792-4607</orcidid><orcidid>https://orcid.org/0000-0001-6102-694X</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1094-4087
ispartof Optics express, 2024-07, Vol.32 (16), p.27278
issn 1094-4087
1094-4087
language eng
recordid cdi_proquest_miscellaneous_3128820794
source DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals
title Calculating point spread functions: methods, pitfalls, and solutions
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-15T06%3A34%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Calculating%20point%20spread%20functions:%20methods,%20pitfalls,%20and%20solutions&rft.jtitle=Optics%20express&rft.au=Holinirina%20Dina%20Miora,%20Ratsimandresy&rft.date=2024-07-29&rft.volume=32&rft.issue=16&rft.spage=27278&rft.pages=27278-&rft.issn=1094-4087&rft.eissn=1094-4087&rft_id=info:doi/10.1364/OE.523532&rft_dat=%3Cproquest_cross%3E3128820794%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3128820794&rft_id=info:pmid/39538569&rfr_iscdi=true