Calculating point spread functions: methods, pitfalls, and solutions
The knowledge of the exact structure of the optical system point spread function (PSF) enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF mode...
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Veröffentlicht in: | Optics express 2024-07, Vol.32 (16), p.27278 |
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creator | Holinirina Dina Miora, Ratsimandresy Rohwer, Erich Kielhorn, Martin Sheppard, Colin Bosman, Gurthwin Heintzmann, Rainer |
description | The knowledge of the exact structure of the optical system point spread function (PSF) enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF models fall into a sampling pitfall near the center position, yielding to the violation of energy conservation. In this work, we present a novel, to the best of our knowledge, Fourier-based techniques for computing vector PSF and compare them to the state-of-the-art. Our methods are shown to satisfy the physical condition of the imaging process. They are reproducible, computationally efficient, easy to implement, and easy to modify to represent various imaging modalities. |
doi_str_mv | 10.1364/OE.523532 |
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title | Calculating point spread functions: methods, pitfalls, and solutions |
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