Calculating point spread functions: methods, pitfalls, and solutions

The knowledge of the exact structure of the optical system point spread function (PSF) enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF mode...

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Veröffentlicht in:Optics express 2024-07, Vol.32 (16), p.27278
Hauptverfasser: Holinirina Dina Miora, Ratsimandresy, Rohwer, Erich, Kielhorn, Martin, Sheppard, Colin, Bosman, Gurthwin, Heintzmann, Rainer
Format: Artikel
Sprache:eng
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Zusammenfassung:The knowledge of the exact structure of the optical system point spread function (PSF) enables a high-quality image reconstruction in fluorescence microscopy. Accurate PSF models account for the vector nature of light and the phase and amplitude modifications. Most existing real-space-based PSF models fall into a sampling pitfall near the center position, yielding to the violation of energy conservation. In this work, we present a novel, to the best of our knowledge, Fourier-based techniques for computing vector PSF and compare them to the state-of-the-art. Our methods are shown to satisfy the physical condition of the imaging process. They are reproducible, computationally efficient, easy to implement, and easy to modify to represent various imaging modalities.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.523532