Characteristics and biological mechanism of protein degradation by the black solider fly (Hermetia illucens L.) larvae gut strain Bacillus subtilis S4

The black solider fly larvae (BSFL) can efficiently convert nitrogen in organic waste into insect protein. Bacillus subtilis S4, an efficient protein-degrading bacterium from the BSFL gut, was isolated and identified to explore the mechanism of nutrient metabolism underlying BSFL nitrogen utilization. Results showed that B. subtilis S4 could effectively increase larval biomass in a bean stem bioconversion system. In vitro high-performance liquid chromatography analysis showed that B. subtilis S4 completely degraded casein into Val, Ile, Phe, Leu, Tyr, Lys, Gly, and Met. Various protease genes with secretion expression ability were annotated in B. subtilis S4. They included peptidoglycan DL - endopeptidase, aminopeptidase, extracellular metalloprotease, peptidoglycan endopeptidase, and peptidase M15. Therefore, the BSFL intestinal microbe B. subtilis S4 could effectively degrade protein and promote larval biomass accumulation, which could provide novel insights into the combined conversion of organic waste into proteins by microbes and insects. [Display omitted] •The BSFL intestinal microbe B. subtilis S4 could effectively degrade protein.•Whole-genome sequencing revealed that Bacillus subtilis S4 has a large number of amino acid metabolism related genes.•The protease secreted by B. subtilis S4 retained desirable activity at 4 °C.•B. subtilis S4 could promote black soldier fly larval biomass accumulation.