Multimodal chemical speciation techniques based on simultaneous high resolution molecular/atomic mass spectrometry applied to online target/non-target analysis: A tutorial review

If identifying target species is challenging regarding chemical speciation, non-target species present even more significant difficulties. Thus, to improve the performance of the methods, multimodal online coupling involving atomic and molecular mass spectrometry (LC-ICP-MS-ESI-HRMS) is an advance in this direction. Then, this kind of coupling is highlighted in this Tutorial Review, as well as some references emphasizing its potentialities and possible limitations. Some crucial definitions of speciomics, chemical speciation, and others are also included. The main parameters that influence the coupling of an inductively coupled plasma mass spectrometer with a high-resolution mass spectrometer through a chromatographic system are critically commented on, and a diversity of results is demonstrated by using a turtle liver (Caretta caretta) as a model sample. The parameters were discussed in detail in a step-by-step manner: ICP-MS/MS acquisition modes and instrumental parameters, HRMS acquisition modes and instrumental parameters, and data processing strategies (Full MS – Top N, All Ion Fragmentation – AIF, Parallel Reaction Monitoring – PRM). Additionally, this Tutorial Review also demonstrates a diversity of results through target and non-target analysis. Constituting a guide for those who are interested in a non-targeted analysis of molecular non-volatile/semi-volatile compounds, this Tutorial Review presents trans and multidisciplinary proposals for those communities involving chemistry, biochemistry, medicine, biology, environmental, pharmaceutical, food safety, and omics, among others, where metal (also metalloids or semi-metals and non-metals or heteroatoms) and molecular species are necessary for a good understanding of the studied system. This kind of coupling also allows the discovery of novel biological active elemental species in diverse matrices. [Display omitted] •Trends in speciomics studies by LC coupled simultaneously with ICP-MS and ESI-HRMS.•A step-by-step guide to multimodal coupling: what is needed for speciomics analysis.•Case study: Target and non-target speciation of As, P and S in real samples.•Tutorial on how to couple HPLC simultaneously to ICP-MS and ESI-HRMS.•Comprehensive strategies for data processing of target and non-target species and novel species.