Installation of an organocatalyst into a protein scaffold creates an artificial Stetterase

Using a protein scaffold covalently functionalised with a thiamine-inspired N-heterocyclic carbene (NHC), we created an artificial Stetterase (ArtiSt) which catalyses a stereoselective, intramolecular Stetter reaction. We demonstrate that ArtiSt functions under ambient conditions with low catalyst l...

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Veröffentlicht in:	Chemical communications (Cambridge, England) England), 2024-11, Vol.6 (93), p.13746-13749
Hauptverfasser:	MacAulay, Alice, Klemencic, Eva, Brewster, Richard C, Ünal, Süleyman Mert, Notari, Evangelia, Wood, Christopher W, Jarvis, Amanda G, Campopiano, Dominic J
Format:	Artikel
Sprache:	eng
Schlagworte:	Chemistry Proteins Scaffolds Stereoselectivity Thiamine
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Using a protein scaffold covalently functionalised with a thiamine-inspired N-heterocyclic carbene (NHC), we created an artificial Stetterase (ArtiSt) which catalyses a stereoselective, intramolecular Stetter reaction. We demonstrate that ArtiSt functions under ambient conditions with low catalyst loading. Furthermore, activity can be increased >20 fold by altering the protein scaffold. Using a protein scaffold covalently functionalised with a thiamine-inspired N-heterocyclic carbene (NHC) we created an artificial Stetterase (ArtiSt) which catalyses a stereoselective, intramolecular Stetter reaction.
ISSN:	1359-7345 1364-548X 1364-548X
DOI:	10.1039/d4cc05182c