Acute sleep loss impairs object but not spatial pattern separation in humans

•Object and spatial pattern separation are differently affected by sleep loss.•Sleep deprivation impaired object pattern separation.•Loss of sleep did not affect spatial pattern separation.•Study supports processing context v. spatial information via different neuronal pathways.•The discrimination projections represent separate but complimentary hippocampal processes. Pattern separation allows us to form discrete representations of information in memory. Pattern separation can be measured in several domains including spatial and object-based discrimination. The brain area largely involved in this process is the dentate gyrus of the hippocampus, which has been shown to be particularly sensitive to the effects of sleep loss. However, methodology in rodent and human studies varies greatly making translational conclusions difficult. Therefore, the aim of the current study was to measure the effects of sleep deprivation on human hippocampal function, using well-validated spatial and object-based pattern separation tests. The effects of acute sleep loss were examined, as this method is frequently used in rodent research but not human studies. Results show that sleep loss impaired performance on the object-based version of the test, but not spatial pattern separation. The findings support the notion that these discrimination projections represent separate but complimentary hippocampal processes, and further elucidates how they may be discretely affected by acute sleep loss.