Description of Millerago gen. nov. based on taxogenomic analysis, with two new species, Millerago phaffii f.a., sp. nov. and Millerago galiae f.a., sp. nov

Four yeast isolates obtained from tree bark and fermenting sap of spp. and insects in Colombia and Japan were phylogenetically related to based on analyses of the sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit rRNA gene. The novel species differs from by 20 nt substitutions and 5 indels in the D1/D2 sequences. A phylogenomic analysis suggested that these species are related to , the genus and a small clade containing , and . Our genomic analyses suggest that the novel species and should be separated in a novel yeast genus. We propose the genus gen. nov. to accommodate these species and the species f.a., sp. nov. (CBS 18021 ; MycoBank MB856172) to accommodate the Colombian and Japanese isolates. The Colombian isolate of differs from the Japanese isolates by three nt substitutions and one indel and two substitutions and one indel in the ITS and D1/D2 sequences, respectively, showing that they were conspecific. We also propose the new species sp. nov. to validate this species according to the rules of the International Code of Nomenclature for algae, fungi and plants.