A vertical/horizontal graphene-based microneedle plant sensor for on-site detection of indole-3-acetic acid in vegetables

Plant hormones are important regulators of crop growth and production. In this study, an in situ electrochemical sensor was successfully built using flat microelectrodes with horizontally and vertically grown graphene to detect the plant regulator indole-3-acetic acid (IAA) in plants. Vertical and horizontal graphene layers were prepared by electron-assisted hot-filament chemical vapor deposition. Vertical graphene nanosheets were grown on a horizontal graphene layer as sensing electrodes, and a microneedle sensor was assembled by combining Pt and Ti microelectrodes. The vertical/horizontal graphene (VHG) microneedle sensor can rapidly detect IAA levels in various plants in situ over a wide pH range of 4.0–9.0 and concentration range of 1–100 μM, with a minimum detection limit of 0.21 μM (3σ/S). Subsequently, this microneedle sensor was used to determine the IAA content in different tissues of cucumber and cauliflower stems with satisfactory results. The combination of VHG microelectrode arrays and small electrochemical workstations is useful for constructing portable, low-cost, on-site, and fast electrochemical sensing platforms for plant growth monitoring. [Display omitted] •The sensing electrodes were composed of vertically and horizontally grown graphene.•Vertical and horizontal graphene synergistically enhance electrochemical activity.•A needle-type plant sensor was assembled for the on-site detection of indole-3-acetic acid.•This small sensor is suitable for monitoring stems, fruits, and leaves of plants.