Water pollutant cadmium-induced intestinal inflammation in Eriocheir sinensis and potential therapeutic drugs

Eriocheir sinensis is a significant crustacean in freshwater aquaculture. However, this industry is vulnerable to diseases, including intestinal inflammation. Cadmium (Cd) is a water pollutant that exacerbates intestinal inflammation in Eriocheir sinensis. This study aimed to investigate the mechanisms of Cd-induced intestinal inflammation in Eriocheir sinensis and to identify potential therapeutic agents. Histology, enzyme activity, and gene expression detection were combined to investigate the promoting effect of Cd on the exacerbation of intestinal inflammation in Eriocheir sinensis. Transcriptome sequencing was performed to analyze the critical signaling pathways regulating intestinal inflammation in Eriocheir sinensis. Transcriptome analysis confirmed that the Toll-like receptor pathway is critical in this process. TLR4-IN-C34, a specific inhibitor of TLR4, was identified as a potential treatment for intestinal inflammation. In vivo results demonstrated that TLR4-IN-C34 effectively reduced the severity of Cd-induced intestinal inflammation in Eriocheir sinensis. The tissue morphology of the intestine, gills, and hepatopancreas of Eriocheir sinensis improved, showing a significant decrease in inflammatory factors and apoptotic genes, along with enhanced antioxidant activity. In conclusion, this study indicates that the water pollutant Cd exacerbates intestinal inflammation in Eriocheir sinensis, and TLR4-IN-C34 regulates the Toll-like receptor pathway in treating intestinal inflammation. This research provides valuable insights into the management of intestinal inflammation and the sustainable cultivation of Eriocheir sinensis. [Display omitted] •Cd exposure exacerbates intestinal inflammation in Eriocheir sinensis.•Toll pathway plays an critical role in Cd-induced intestinal inflammation in Eriocheir sinensis.•TLR4-IN-C34 effectively reduces the severity of Cd-induced intestinal inflammation in Eriocheir sinensis.