Pretreated polystyrene is degraded by a microbial consortium enriched from wetland plastic waste

The biodegradation of polystyrene (PS), a type of plastic with aromatic rings in its polymer chain, is a critical environmental goal worldwide. Microbial degradation of PS has been reported, but the underlying mechanisms are poorly understood. Here, we constructed a microcosm wetland containing PS plastic. We isolated six highly efficient PS plastic–degrading bacterial strains and created a microbial consortium (MCs) consisting of these strains. After a 30-day incubation period, MCs-treated PS exhibited hallmarks of degradation, including –CO– formation, reduced hydrophobicity, surface porosity, and 20 % weight loss. The efficiency of PS degradation was enhanced by using a combination of physical-chemical pretreatment and biological methods, increasing the microbial degradation rate by 20 %. Antioxidant 2246 (C23H32O2) was detected in the culture supernatant via GC-MS. Metatranscriptomic sequencing analysis provided insight into the possible metabolic pathway of PS degradation by the composite bacteria. We identified 31 highly expressed genes encoding proteins that function in carbon metabolism pathways and 34 unique proteases which catalyze the cleavage of long polymer chains. The resulting small molecules are absorbed and further degraded intracellularly by enzymes such as coenzyme synthase, hydratase, transferase, carboxylase, and dehydrogenase. These findings lay the foundation for the efficient and sustainable degradation of PS. [Display omitted] •Enrichment of microbial species were discrepant with different plastic sizes.•Synergistically acting bacteria form efficient biodegrading microbiome.•Pretreatment enhanced the ability of microbial consortium to degrade polystyrene.•Differences metabolic pathways of polystyrene-degrading bacteria were revealed.