Degradation of pathogenic amyloids induced by matrix metalloproteinase-9

Over the past decade, the greatest promise for treating severe and currently incurable systemic and neurodegenerative diseases has turned to agents capable of effectively degrading pathological amyloid deposits without causing side effects. Specifically, amyloid destruction observed in immunotherapy is hypothesized to occur through activation of proteolytic enzymes. This study examines poorly understood effects of an immune enzyme, extracellular matrix metalloproteinase-9 (MMP9), on amyloids associated with Alzheimer's and Parkinson's diseases, lysozyme, insulin, and dialysis-related amyloidoses. The study establishes the universality of MMP9's effect on various amyloids, with its efficacy largely depending on the fibrillar cluster size. Irreversible amyloid degradation by MMP9 is attributed to the destruction of intramolecular interactions rather than intermolecular hydrogen bonds in the fibril backbone. This process results in the loss of ordered fiber structure without reducing aggregate size or increasing cytotoxicity. Thus, MMP9 can mitigate side effects of anti-amyloid therapy associated with the formation of low-molecular-weight degradation products that may accelerate fibrillogenesis and amyloid propagation between tissues and organs. MMP9 shows promise as a component of safe anti-amyloid drugs by enhancing the accessibility of binding sites through “loosening” amyloid clusters, which facilitates subsequent fragmentation and monomerization by other enzymes. •MMP9 mediates an irreversible loss of ordered structure in amyloid fibrils without fragmentation.•Amyloid degradation by MMP9 involves destabilizing intramolecular interactions rather than intermolecular hydrogen bonds.•Efficiency of MMP9-induced degradation depends on the accessibility of enzyme binding sites on amyloid fibrils.•MMP9 treatment can reduce amyloid cytotoxicity, in contrast to its usual exacerbation by other exposures.•MMP9-induced amyloid pre-loosening combined with other enzyme-mediated fragmentation offers a safe therapeutic strategy.