A method for quantitative and base-resolution sequencing of pseudouridine

A method for quantitative and base-resolution sequencing of pseudouridine

Quantitative and sensitive methods for the detection of pseudouridine (Ψ) have been lacking. Now, a method termed 2-bromoacrylamide-assisted cyclization sequencing (BACS) has been developed that enables the accurate quantification of Ψ stoichiometry, precise identification of Ψ positions and robust...

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Veröffentlicht in:Nature methods 2024-11, Vol.21 (11), p.1984-1985
Format: Artikel
Sprache:eng
Schlagworte:
631/61/514/1949
631/92/500
Adenosine
Bioinformatics
Biological Microscopy
Biological Techniques
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Enzymes
Life Sciences
Methods
Proteomics
Research Briefing
Stoichiometry
Transfer RNA
Viruses
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Beschreibung
Zusammenfassung:Quantitative and sensitive methods for the detection of pseudouridine (Ψ) have been lacking. Now, a method termed 2-bromoacrylamide-assisted cyclization sequencing (BACS) has been developed that enables the accurate quantification of Ψ stoichiometry, precise identification of Ψ positions and robust detection of densely modified Ψ sites.
ISSN:1548-7091
1548-7105
1548-7105
DOI:10.1038/s41592-024-02466-5