Urinary tract infection: Conventional testing to developing Technologies

•Reviewed the epidemiology, biomarker, conventional & developing diagnostics for UTI.•The review investigates the potential of conventional and developing biomarkers.•Biofilm-centric modalities will advance the landscape of clinical diagnostics.•Use of microfluidics-integrated biosensor has increased the diagnostic efficiency. Urinary tract infections (UTIs) present an escalating global health concern, precipitating increased hospitalizations and antibiotic utilization, thereby fostering the emergence of antimicrobial resistance. Current diagnostic modalities exhibit protracted timelines and substantial financial burdens, necessitating specialized infrastructures. Addressing these impediments mandates the development of a precise diagnostic paradigm to expedite identification and augment antibiotic stewardship. The application of biosensors, recognized for their transformative efficacy, emerges as a promising resolution. Recent strides in biosensor technologies have introduced pioneering methodologies, yielding pertinent biosensors and integrated systems with significant implications for point-of-care applications. This review delves into historical perspectives, furnishing a comprehensive delineation of advancements in UTI diagnostics, disease etiology, and biomarkers, underscoring the potential merits of these innovations for optimizing patient care.