Paper-based multicolor sensor for on-site quantitative detection of organophosphate pesticides based on acetylcholinesterase-mediated paper-based Au3+-etching of gold nanobipyramids and CIELab color space

On-site quantitative detection of organophosphorus pesticides (OPs) is crucial for safeguarding food and public safety. This study presents a novel acetylcholinesterase (AChE)-mediated paper-based Au3+-etching of gold nanobipyramids (AuNBPs) system. The system employs a long-term storable AuNBPs-dep...

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Veröffentlicht in:Talanta (Oxford) 2025-01, Vol.281, p.126925, Article 126925
Hauptverfasser: Zhang, Feng, Gao, Yu, Ren, Enxi, Fang, Ling, Yang, Weijuan, Zhang, Liaoyuan, Wang, Zongwen
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Sprache:eng
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Zusammenfassung:On-site quantitative detection of organophosphorus pesticides (OPs) is crucial for safeguarding food and public safety. This study presents a novel acetylcholinesterase (AChE)-mediated paper-based Au3+-etching of gold nanobipyramids (AuNBPs) system. The system employs a long-term storable AuNBPs-deposited nylon membrane embedded within a portable and temperature-controlled paper-based analytical device. This system, coupled with a colorimeter-based quantitative method, enables the development of a practical paper-based multicolor sensor (PMS) for on-site quantitative detection of three common OPs (paraoxon, dichlorvos, and trichlorfon). In the absence of OPs, AChE hydrolyzes acetylthiocholine to thiocholine, which reduces Au3+ to Au+. The presence of OPs inhibits AChE activity, thereby preserving Au3+ to etch AuNBPs on nylon membranes, accompanied by multicolor changes. These color changes can be simply quantified by measuring the a∗ parameter of the CIELab color space using a portable colorimeter. Under optimal conditions, the PMS displayed eight OPs-corresponding color changes with a minimum detectable concentration of 1.0–10 μg/L (visual observation) and limits of detection of 0.8–7.2 μg/L (colorimeter) and 0.2–3.4 μg/L (UV–vis spectrometry). The PMS successfully determined the OPs in vegetable and rice samples with recoveries of 89.0–109 % and RSDs (n = 5) of
ISSN:0039-9140
1873-3573
1873-3573
DOI:10.1016/j.talanta.2024.126925