Copaifera spp. oleoresins control Trypanosoma cruzi infection in human trophoblast cells (BeWo) and placental explants

Congenital Chagas disease (CCD) is a worldwide neglected problem with significant treatment limitations. This study aimed to evaluate the potential of Copaifera spp. oleoresins (ORs) against Trypanosoma cruzi infection in trophoblast cells (BeWo lineage) and human chorionic villous explants (HCVE)....

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Veröffentlicht in:Biomedicine & pharmacotherapy 2024-10, Vol.179, p.117425, Article 117425
Hauptverfasser: Júnior, Joed Pires de Lima, Teixeira, Samuel Cota, de Souza, Guilherme, Faria, Guilherme Vieira, Almeida, Marcos Paulo Oliveira, Franco, Priscila Silva, Luz, Luana Carvalho, Paschoalino, Marina, dos Santos, Natália Carine Lima, de Oliveira, Rafael Martins, Martínez, Aryani Felixa Fajardo, Rosini, Alessandra Monteiro, Ambrosio, Maria Anita Lemos Vasconcelos, Veneziani, Rodrigo Cassio Sola, Bastos, Jairo Kenupp, Gomes, Angelica Oliveira, Alves, Rosiane Nascimento, da Silva, Claudio Vieira, Martins, Carlos Henrique Gomes, Ferro, Eloisa Amália Vieira, Barbosa, Bellisa Freitas
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Sprache:eng
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Zusammenfassung:Congenital Chagas disease (CCD) is a worldwide neglected problem with significant treatment limitations. This study aimed to evaluate the potential of Copaifera spp. oleoresins (ORs) against Trypanosoma cruzi infection in trophoblast cells (BeWo lineage) and human chorionic villous explants (HCVE). The cytotoxicity of ORs was investigated using LDH and MTT assays. T. cruzi (Y strain) proliferation, invasion and reversibility were assessed in OR-treated BeWo cells, and proliferation was evaluated in OR-treated HCVE. The ultrastructure of T. cruzi trypomastigotes and amastigotes treated with ORs were analyzed by scanning and transmission electronic microscopy. ROS production in infected and treated BeWo cells and cytokines in BeWo and HCVE were measured. The ORs irreversibly decreased T. cruzi invasion, proliferation and release in BeWo cells by up to 70 %, 82 % and 80 %, respectively, and reduced parasite load in HCVE by up to 80 %. Significant structural changes in treated parasites were observed. ORs showed antioxidant capacity in BeWo cells, reducing ROS production induced by T. cruzi infection. Also, T. cruzi infection modulated the cytokine profile in both BeWo cells and HCVE; however, treatment with ORs upregulated cytokines decreased by T. cruzi infection in BeWo cells, while downregulated cytokines increased by the T. cruzi infection in HCVE. In conclusion, non-cytotoxic concentrations of Copaifera ORs demonstrated promising potential for controlling T. cruzi infection in models of the human maternal-fetal interface. [Display omitted]
ISSN:0753-3322
1950-6007
1950-6007
DOI:10.1016/j.biopha.2024.117425